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Published on: May 28, 2015
Generation of a homozygous TIGIT gene knockout (TIGIT-/-) human iPSC line (MUSIi001-A-3) using CRISPR/Cas9 system
Jakkrapatra Srisantitham1, Siriwal Suwanpitak2, Nontaphat Thongsin2
1Siriraj Center for Regenerative Medicine, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Adoptive cell therapy for solid cancers involves enhancing and reinfusing immune cells to target tumor cells. The advancement of induced pluripotent stem cell technology enables the generation of immune cell products like T and NK cells for ACT. However, the expression of inhibitory receptors, such as TIGIT, may limit the functionality of these immune effector cells. In this study, we generated a homozygousTIGITgene knockout iPSC line to potentially prevent inhibitory signaling and exhaustion, thereby creating potent "off-the-shelf" immune cell products for cellular immunotherapy applications. This approach could offer a new frontier in the fight against solid tumors.
Adoptive cell therapy for solid cancers involves enhancing and reinfusing immune cells to target tumor cells. The advancement of induced pluripotent stem cell technology enables the generation of immune cell products like T and NK cells for ACT. However, the expression of inhibitory receptors, such as TIGIT, may limit the functionality of these immune effector cells. In this study, we generated a homozygousTIGITgene knockout iPSC line to potentially prevent inhibitory signaling and exhaustion, thereby creating potent "off-the-shelf" immune cell products for cellular immunotherapy applications. This approach could offer a new frontier in the fight against solid tumors.
