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Updated: Jun 9, 2025

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
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Human prolyl hydroxylase domain 2 reacts with O2 and 2-oxoglutarate to enable formation of inactive

Giorgia Fiorini1, Stephen A Marshall1, William D Figg1

  • 1Chemistry Research Laboratory, Department of Chemistry and the Ineos Oxford Institute for Antimicrobial Research, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK.

Scientific Reports
|October 31, 2024
PubMed

Keywords:
EpigeneticsHypoxia inducible factorHypoxia/oxygen sensingL-Ascorbic acid/vitamin CProlyl hydroxylase domain (PHD)/EGLN enzymeTranscriptional regulationα-Ketoglutarate/2-oxoglutarate oxygenase

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