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Related Concept Videos

  1. Home
  3. Research Domains
  5. Agricultural, Veterinary And Food Sciences
  7. Agricultural Biotechnology
  9. Agricultural Biotechnology Diagnostics (incl. Biosensors)
  11. Resistance Of Anopheles Gambiae S.s. Against Commonly Used Insecticides And Implication Of Cytochrome P450 Monooxygenase In Resistance To Pyrethroids In Lambaréné (gabon).
  1. Home
  3. Research Domains
  5. Agricultural, Veterinary And Food Sciences
  7. Agricultural Biotechnology
  9. Agricultural Biotechnology Diagnostics (incl. Biosensors)
  11. Resistance Of Anopheles Gambiae S.s. Against Commonly Used Insecticides And Implication Of Cytochrome P450 Monooxygenase In Resistance To Pyrethroids In Lambaréné (gabon).

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Resistance of Anopheles gambiae s.s. against commonly used insecticides and implication of cytochrome P450 monooxygenase in resistance to pyrethroids in Lambaréné (Gabon).

Stravensky Térence Boussougou-Sambe1,2, Ynous Djida3, Ange Gatien Doumba-Ndalembouly3

  • 1Centre de Recherches Médicales de Lambaréné, Lambaréné, P.O. Box 242, Gabon. stravsambe2@gmail.com.

BMC Infectious Diseases
|October 31, 2024

View abstract on PubMed

Summary
This summary is machine-generated.
Keywords:
A. gambiae s.s.Cytochrome p450GabonPyrethroids

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Insecticide resistance in Anopheles gambiae mosquitoes threatens malaria control. P450 enzymes contribute to pyrethroid resistance, suggesting combination therapies for effective vector control.

Area of Science:

  • Vector Control
  • Medical Entomology
  • Molecular Entomology

Background:

  • Insecticide resistance in Anopheles mosquitoes poses a significant threat to malaria vector control efforts.
  • Limited data on phenotypic insecticide resistance in Gabon hinders effective resistance management strategies.

Purpose of the Study:

  • To assess the susceptibility of Anopheles gambiae sensu lato to pyrethroids, carbamates, and organophosphates in Lambaréné, Gabon.
  • To investigate the mechanisms underlying pyrethroid resistance in Anopheles vectors.

Main Methods:

  • Larval collection and adult rearing of Anopheles gambiae s.l. from Lambaréné.
  • WHO tube assays using diagnostic and elevated insecticide concentrations, with and without piperonyl butoxide (PBO).
  • Molecular screening for target-site resistance mutations in the Voltage-gated sodium channel (Vgsc) and acetylcholinesterase (Ace-1) genes.
Resistance intensity

Main Results:

  • Anopheles gambiae sensu stricto exhibited high resistance to permethrin and deltamethrin, but susceptibility to malathion.
  • Resistance to bendiocarb was observed, while the Ace-1 G119S mutation was absent.
  • Piperonyl butoxide significantly enhanced pyrethroid efficacy, implicating cytochrome P450 monooxygenases in resistance.
  • Pyrethroid resistance was associated with Vgsc-1014 mutations (L1014F or L1014S).

Conclusions:

  • Cytochrome P450 monooxygenases play a key role in pyrethroid resistance in Anopheles gambiae s.s. from Lambaréné.
  • Combining PBO with pyrethroids, as in second-generation bednets, could help overcome resistance.
  • Malathion offers a potential alternative or complementary agent for insecticide resistance management strategies.