Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Associations between the Plasmodium falciparum genome and sickle haemoglobin identified in mild malaria cases from Ghana.

Malaria journal·2026
Same author

Plasmodium falciparum PfMyoF contains a Rab-like tail domain and associates with perinuclear membrane trafficking proteins.

Communications biology·2026
Same author

In vivo reprogramming of cytotoxic effector CD8 T cells via fractalkine-conjugated mRNA-LNPs.

Science immunology·2026
Same author

GravSorter: a forward-genetics tool for studying gravity response in <i>Caenorhabditis elegans</i>.

The Analyst·2026
Same author

The fascinating malaria-COVID-19 relationship.

The Lancet. Global health·2026
Same author

Addressing pandemic-wide systematic errors in the SARS-CoV-2 phylogeny.

Nature methods·2026

Related Experiment Video

Updated: Jun 9, 2025

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

17.1K

Self-actuated microfluidic chiplet for two-stage multiplex nucleic acid amplification assay.

Felix Ansah1,2, Marziyeh Hajialyani1, Fatemeh Ahmadi1

  • 1Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania, 233 Towne Building, Philadelphia, Pennsylvania 19104, USA. bau@seas.upenn.edu.

Lab on a Chip
|October 31, 2024
PubMed
Summary

A novel self-actuated chiplet enables point-of-need, multiplexed pathogen detection using the Penn-RAMP assay. This device efficiently differentiates co-endemic diseases for precision medicine and improved public health control.

More Related Videos

Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

7.2K
Microfluidic Chip Fabrication and Method to Detect Influenza
09:43

Microfluidic Chip Fabrication and Method to Detect Influenza

Published on: March 26, 2013

14.9K

Related Experiment Videos

Last Updated: Jun 9, 2025

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

17.1K
Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

7.2K
Microfluidic Chip Fabrication and Method to Detect Influenza
09:43

Microfluidic Chip Fabrication and Method to Detect Influenza

Published on: March 26, 2013

14.9K

Area of Science:

  • Biotechnology
  • Molecular Diagnostics
  • Point-of-Care Testing

Background:

  • Accurate diagnosis of diseases with overlapping symptoms requires assays that can detect multiple pathogens simultaneously.
  • Existing methods often lack the speed and specificity needed for timely clinical decisions.
  • The Penn-RAMP assay was developed for multiplexed, isothermal amplification to address these diagnostic challenges.

Purpose of the Study:

  • To develop a self-actuated chiplet for the Penn-RAMP assay, enabling point-of-need pathogen detection.
  • To integrate automated sample handling and multiplexed amplification onto a single, user-friendly device.
  • To demonstrate the chiplet's capability for co-detection and differentiation of multiple targets.

Main Methods:

  • Development of a self-actuated chiplet incorporating temperature-controlled phase change and capillary valves.
  • Integration of a two-stage isothermal amplification process: recombinase polymerase amplification (RPA) followed by multiplexed loop-mediated isothermal amplification (LAMP).
  • Automated self-aliquotting of RPA amplicons into five distinct LAMP reaction chambers for target-specific amplification.

Main Results:

  • The chiplet successfully demonstrated co-detection of plant pathogens, validating its functionality.
  • Analytical performance of the chiplet was comparable to the benchtop Penn-RAMP assay.
  • The chiplet outperformed standalone LAMP assays in terms of sensitivity and specificity.

Conclusions:

  • The self-actuated Penn-RAMP chiplet provides a robust platform for point-of-need, multiplexed nucleic acid amplification and detection.
  • This technology facilitates precision medicine by enabling rapid differentiation of co-endemic pathogens.
  • The chiplet can be used independently or integrated into broader sample preparation workflows for diverse diagnostic applications.