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Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Updated: Jun 9, 2025

Synthesis and Bioconjugation of Thiol-Reactive Reagents for the Creation of Site-Selectively Modified Immunoconjugates

Synthesis and Bioconjugation of Thiol-Reactive Reagents for the Creation of Site-Selectively Modified Immunoconjugates

Published on: March 6, 2019

Boron enabled bioconjugation chemistries.

Mengmeng Zheng¹, Lingchao Kong¹, Jianmin Gao¹

¹Department of Chemistry, Merkert Chemistry Center, Boston College, 2609 Beacon Street, Chestnut Hill, MA 02467, USA. jianmin.gao@bc.edu.

Chemical Society Reviews

|October 31, 2024

Summary

This summary is machine-generated.

Boron-enabled bioconjugation reactions offer unique biocompatibility and tunable reversibility. This review analyzes boron

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Area of Science:

Chemistry
Bioconjugation Chemistry
Materials Science

Background:

Bioconjugation reactions are crucial for molecular labeling and material construction.
Boron's unique properties, including biocompatibility, have led to novel bioconjugation strategies.
Existing methods often lack tunable reversibility or broad bioorthogonal applicability.

Purpose of the Study:

To summarize and comparatively analyze recent advancements in boron-mediated bioconjugations.
To discuss the thermodynamic and kinetic profiles of boron-enabled reactions.
To provide guidelines for applying these reactions in various bioapplications.

Main Methods:

Review and analysis of recently developed boron-mediated bioconjugation reactions.
Comparative assessment of thermodynamic and kinetic properties.
Exploration of structure-reactivity relationships in organoboron reagents.

Main Results:

Boron-enabled bioconjugations demonstrate remarkable biocompatibility and tunable reversibility.
Rapid reversible and stable covalent bioconjugations have been achieved.
These reactions expand the scope of bioorthogonal chemistry and reversible covalent inhibition.

Conclusions:

Boron-mediated chemistry offers a versatile platform for developing advanced bioconjugation strategies.
Understanding thermodynamic and kinetic profiles is key to optimizing reaction design.
These findings provide valuable insights for future applications in chemical biology and materials science.