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Related Concept Videos

Cell Migration01:09

Cell Migration

Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.

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Nuclear deformability facilitates apical nuclear migration in the developing zebrafish retina.

Mariana Maia-Gil1, Maria Gorjão1, Roman Belousov2

  • 1Gulbenkian Institute for Molecular Medicine (GIMM) (previously Instituto Gulbenkian de Ciência), Rua da Quinta Grande 6, 2780-156 Oeiras, Portugal.

Current Biology : CB
|October 31, 2024
PubMed
Summary

Nuclear stiffness, influenced by lamin A expression, hinders apical nuclear migration in developing zebrafish tissues. These effects are tissue-dependent and can occur independently of direct nuclear lamin A levels.

Keywords:
Lamin A/Cnuclear migrationnuclear propertiespseudostratified neuroepitheliazebrafish

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Area of Science:

  • Cell Biology
  • Developmental Biology
  • Biophysics

Background:

  • Nuclear positioning is vital for cell and tissue development.
  • Apical nuclear migration in neuroepithelia is critical for proper tissue formation.
  • The role of nuclear properties, like deformability, in this migration is less understood.

Purpose of the Study:

  • To investigate how lamin A expression affects nuclear deformability and apical migration in zebrafish neuroepithelia.
  • To determine if nuclear material properties influence migration in a tissue-specific manner.

Main Methods:

  • Overexpression of lamin A in zebrafish retinal neuroepithelial cells.
  • Assessment of nuclear stiffness and deformability.
  • Observation of apical nuclear migration during mitosis.
  • Comparison between retinal and hindbrain neuroepithelia.

Main Results:

  • Increased lamin A expression stiffens nuclei, impairing apical migration.
  • Cell non-autonomous effects observed: nuclei in a stiffer environment showed impaired migration.
  • Less pronounced effects in the less crowded hindbrain neuroepithelium.
  • Nuclear material properties influence migration in a tissue-dependent manner.

Conclusions:

  • Nuclear stiffness, modulated by lamin A, is a key factor in apical nuclear migration.
  • Tissue environment and density play a role in nuclear positioning.
  • Findings highlight the interplay between nuclear properties and tissue context in development.