Optimized full-spectrum flow cytometry panel for deep immunophenotyping of murine lungs

Area of Science:

• Immunology
• Cancer Metastasis
• Flow Cytometry

Background:

• The lung immune system is crucial for defense against pathogens and cancer.
• Assessing lung immune cell changes is vital for understanding infectious diseases, primary lung cancer, and metastasis.
• High autofluorescence in lung tissue complicates high-parameter flow cytometry analysis.

Purpose of the Study:

• To develop an optimized antibody panel for comprehensive immune cell analysis in murine lungs.
• To overcome the challenge of lung tissue autofluorescence in spectral flow cytometry.
• To enable quantitative assessment of immune cell populations in normal and metastatic lung environments.

Main Methods:

• Development of a 30-parameter antibody panel.
• Application of spectral flow cytometry for immune cell analysis.
• Analysis of immune cell types and states in normal and metastatic murine lungs.

Main Results:

• An optimized 30-parameter antibody panel was successfully established.
• The panel enables detailed analysis of major immune cell populations and states.
• Effective characterization of immune cells in both normal and metastatic murine lungs was achieved.

Conclusions:

• The optimized antibody panel enhances the analysis of lung immunity using spectral flow cytometry.
• This tool is valuable for studying immune responses in infectious diseases, lung cancer, and metastasis.
• The panel facilitates a deeper understanding of lung immune cell dynamics in pathological conditions.