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TNFRSF11B promotes the progression of bladder cancer through PI3K/AKT signaling pathway

  • 0Department of Urology, Renmin Hospital of Wuhan University, Wuhan, 430060, China; Department of Urology, The First Affiliated Hospital of Yangtze University, Jingzhou, 434000, China.
Molecular and Cellular Probes +

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October 31, 2024

|

October 31, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

TNFRSF11B promotes bladder cancer (BC) growth and spread by activating the PI3K/AKT pathway. This protein is upregulated in BC and may serve as a prognostic marker for treatment.

Area Of Science

  • Oncology
  • Molecular Biology
  • Cancer Research

Background

  • Tumorigenesis is influenced by TNFRSF11B in various cancers.
  • The role and mechanism of TNFRSF11B in bladder cancer (BC) remain largely unexplored.
  • Understanding TNFRSF11B's function is crucial for BC progression and treatment strategies.

Purpose Of The Study

  • To investigate the clinical significance and signaling pathway of TNFRSF11B in bladder cancer.
  • To elucidate the tumorigenic mechanism of TNFRSF11B in BC progression.
  • To evaluate TNFRSF11B as a potential prognostic biomarker for BC.

Main Methods

  • Bioinformatic analysis was employed to assess TNFRSF11B expression and its clinical significance in BC.
  • Functional experiments, including proliferation, migration, invasion, and apoptosis assays, were performed on BC cells.
  • Western blotting was used to analyze epithelial-mesenchymal transition (EMT) markers and PI3K/AKT pathway molecules. In vivo assays and pathway inhibition studies (LY294002) were conducted.

Main Results

  • TNFRSF11B was significantly upregulated in both BC tissues and cell lines.
  • Inhibition of TNFRSF11B suppressed BC cell proliferation, migration, and invasion, while overexpression promoted these malignant traits.
  • TNFRSF11B promotes BC progression by activating the PI3K/AKT pathway, as confirmed by in vivo studies and pathway inhibitor experiments.

Conclusions

  • TNFRSF11B plays a significant tumor-promoting role in bladder cancer through the PI3K/AKT signaling pathway.
  • Elevated TNFRSF11B expression is associated with increased malignancy in BC.
  • TNFRSF11B represents a potential valuable prognostic biomarker for bladder cancer treatment.

Keywords:

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