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Updated: Jun 9, 2025

Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

Comprehensive circular RNA profiling in various sheep tissues.

Mohammad Reza Bakhtiarizade¹, Maryam Heidari², Amir Hossein Mohammadi Ghanatghestani³

¹Department of Animal and Poultry Science, College of Aburaihan, University of Tehran, Tehran, Iran. mrbakhtiari@ut.ac.ir.

Scientific Reports

|November 1, 2024

Summary

This summary is machine-generated.

This study maps circular RNA (circRNA) expression across 12 sheep tissues, identifying 8,831 high-confidence circRNAs, including 88 novel ones. Tissue-specific circRNAs suggest roles in modulating physiological functions.

Keywords:

Functional enrichment analysis Non-coding RNA RNA-Seq Tissue-specific lncRNAs

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Last Updated: Jun 9, 2025

Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

Published on: November 28, 2018

Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species

Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species

Published on: May 19, 2018

Area of Science:

Genomics and Molecular Biology
Animal Science
Bioinformatics

Background:

Circular RNAs (circRNAs) are crucial but understudied in sheep.
Previous sheep circRNA research focused on limited tissues, lacking comprehensive expression profiling.
Understanding circRNA expression is vital for sheep genomics and veterinary research.

Purpose of the Study:

To comprehensively identify and profile circRNA expression across 12 diverse sheep tissues.
To investigate the characteristics and tissue-specificity of sheep circRNAs.
To explore the functional implications of circRNAs in sheep physiology.

Main Methods:

Uniform analysis of 61 public RNA sequencing datasets from 12 sheep tissues.
Application of a stringent computational pipeline for circRNA identification and characterization.
Tissue-specificity, co-expression, and functional enrichment analyses were performed.

Main Results:

Identification of 8,919 high-confidence exon-derived circRNAs, including 88 novel circRNAs.
Discovery of 3,059 tissue-specific circRNAs, with higher specificity than linear RNAs.
Host genes of tissue-specific circRNAs were enriched in functions relevant to their respective tissues, indicating regulatory roles.

Conclusions:

This study presents the first comprehensive circRNA expression landscape in sheep, including novel tissues.
Tissue-specific circRNAs play significant roles in modulating physiological activities across different sheep tissues.
The findings provide a valuable resource for sheep genomic and veterinary research, highlighting common regulatory mechanisms.