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Updated: Apr 28, 2026

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
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SLB-msSIM: a spectral library-based multiplex segmented SIM platform for single-cell proteomic analysis.

Lakmini Senavirathna, Cheng Ma, Van-An Duong

    Biorxiv : the Preprint Server for Biology
    |November 1, 2024
    PubMed
    Summary
    This summary is machine-generated.

    We developed a new method, spectral library-based multiplex segmented selected ion monitoring (SLB-msSIM), for highly sensitive single-cell proteomics. This robust approach enables detailed analysis of cellular heterogeneity and trajectories.

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    Area of Science:

    • Proteomics
    • Mass Spectrometry
    • Cell Biology

    Background:

    • Single-cell proteomics is crucial for understanding cellular heterogeneity.
    • Existing methods face challenges in sensitivity and robustness.

    Purpose of the Study:

    • To develop a sensitive and robust method for single-cell proteomics.
    • To enable detailed analysis of cellular heterogeneity and dynamics.

    Main Methods:

    • Developed the spectral library-based multiplex segmented selected ion monitoring (SLB-msSIM) method.
    • Acquired single-cell mass spectrometry data using the msSIM technique.
    • Utilized spectral matching with a defined spectral library for proteomic identification.

    Main Results:

    • Demonstrated significantly enhanced sensitivity and robustness for single-cell analysis.
    • Successfully interrogated cellular heterogeneity across multiple cell lines.
    • Analyzed cellular trajectories during epithelial-mesenchymal transition.

    Conclusions:

    • SLB-msSIM is a highly sensitive and robust platform for single-cell proteomic studies.
    • The method facilitates the investigation of cellular heterogeneity and functional phenotypes.