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F-BAR proteins CIP4 and FBP17 function in cortical neuron radial migration and process outgrowth
Lauren A English1, Russell J Taylor2, Connor J Cameron2
1Neuroscience Training Program, School of Medicine and Public Health, University of Wisconsin, Madison, WI 53705.
Biorxiv : the Preprint Server for Biology
|November 1, 2024
Summary
The F-BAR proteins CIP4 and FBP17 are crucial for regulating neurite outgrowth during radial neuron migration in the developing cortex. Their modulation disrupts neuronal migration and morphology, highlighting their essential role in cortical development.
Area of Science:
- Neuroscience
- Developmental Biology
- Cell Biology
Background:
- Neurite initiation is vital for neuronal differentiation and migration.
- Neuronal migration involves dynamic neurite extension/retraction, transitioning between bipolar and multipolar states.
- Regulation of neurite dynamics during migration is not well understood.
Purpose of the Study:
- To investigate the in vivo function of F-BAR proteins CIP4 and FBP17 in radial neuron migration and differentiation.
- To determine how CIP4 and FBP17 regulate neurite extension and retraction during cortical development.
Main Methods:
- Utilized in utero electroporation and the Double UP technique in mouse models.
- Compared knockdown or overexpression of CIP4 and FBP17 with control cells within the same tissue.
- Analyzed effects on neuronal morphology and radial migration.
Main Results:
- Modulating CIP4 and FBP17 expression (knockdown or overexpression) significantly disrupted radial neuron migration.
- Changes in CIP4 and FBP17 levels altered neuronal morphology and neurite outgrowth.
- In vivo findings were consistent with previous in vitro observations.
Conclusions:
- F-BAR proteins CIP4 and FBP17 are essential regulators of radial neuron migration in the developing cortex.
- These proteins play a critical role in controlling neuronal morphology and neurite dynamics during migration.
- CIP4 and FBP17 are key players in mammalian cortical development.
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