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Related Concept Videos

Responses to Salt Stress02:02

Responses to Salt Stress

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Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
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Transcription01:10

Transcription

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Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
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Global Regulatory Systems01:28

Global Regulatory Systems

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Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
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Responses to Heat and Cold Stress02:45

Responses to Heat and Cold Stress

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Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
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Combinatorial Gene Control02:33

Combinatorial Gene Control

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Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
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Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

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Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
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  9. Crop And Pasture Biochemistry And Physiology
  11. Expression Interplay Of Genes Coding For Calcium-binding Proteins And Transcription Factors During The Osmotic Phase Provides Insights On Salt Stress Response Mechanisms In Bread Wheat.
  1. Home
  3. Research Domains
  5. Agricultural, Veterinary And Food Sciences
  7. Crop And Pasture Production
  9. Crop And Pasture Biochemistry And Physiology
  11. Expression Interplay Of Genes Coding For Calcium-binding Proteins And Transcription Factors During The Osmotic Phase Provides Insights On Salt Stress Response Mechanisms In Bread Wheat.

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Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress
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Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress

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Expression interplay of genes coding for calcium-binding proteins and transcription factors during the osmotic phase provides insights on salt stress response mechanisms in bread wheat.

Diana Duarte-Delgado1,2,3, Inci Vogt1, Said Dadshani1

  • 1INRES-Plant Breeding, University of Bonn, Bonn, Germany.

Plant Molecular Biology
|November 1, 2024

View abstract on PubMed

Summary
This summary is machine-generated.
Keywords:
3ʹ-UTRCa2+ signalingEF-hand domainExpression profiles

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Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis
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Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis

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Forward Genetic Screen Using Transgenic Calcium Reporter Aequorin to Identify Novel Targets in Calcium Signaling

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Related Experiment Videos

Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress
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Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis
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Forward Genetic Screen Using Transgenic Calcium Reporter Aequorin to Identify Novel Targets in Calcium Signaling
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Forward Genetic Screen Using Transgenic Calcium Reporter Aequorin to Identify Novel Targets in Calcium Signaling

Published on: August 1, 2020

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Soil salinization impacts wheat yield. Calcium signaling via calcium-binding proteins and transcription factors (TFs) is key for salt tolerance, with EF-hand proteins aiding acclimation in tolerant wheat varieties.

Area of Science:

  • Plant Biology
  • Molecular Biology
  • Agronomy

Background:

  • Soil salinization is a major threat to global food security, significantly reducing crop yields, particularly for essential crops like bread wheat.
  • Calcium (Ca2+) signaling plays a critical role in plant acclimation to osmotic stress, mediating early responses through calcium-sensing proteins and transcription factors (TFs).

Purpose of the Study:

  • To comprehensively understand the salt stress response in bread wheat by integrating physiological, genetic, and transcriptomic data.
  • To investigate the role of calcium-binding proteins and their functional diversity in differential salt tolerance between susceptible and tolerant wheat genotypes.

Main Methods:

  • Integrated analysis of physiological, genetic mapping, and transcriptomics data from contrasting wheat genotypes (Syn86 and Zentos).
Osmotic phase
Polymorphisms
Promoter
Sal stress transcriptome
  • Massive Analysis of cDNA 3'-Ends (MACE) based transcriptome analysis to identify salt-responsive genes within 4 hours of stress exposure.
  • Functional and structural diversity analysis of calcium-binding proteins and their expression patterns.

    • Main Results:

    • Salt-responsive genes, particularly those encoding calcium-binding proteins, were over-represented in the transcriptome analysis.
    • The non-EF-hand calcium-binding proteins were associated with salt susceptibility, while EF-hand domain proteins (e.g., RBOHD orthologs) and TFs were rapidly and highly upregulated in the salt-tolerant genotype.
    • Single nucleotide polymorphisms (SNPs) were identified in promoter and 3'-UTR regions, suggesting mechanisms for differential gene expression via TF binding or mRNA stability.

    Conclusions:

    • The differential expression of calcium-binding proteins, particularly EF-hand versus non-EF-hand types, is crucial for salt tolerance in wheat.
    • Interactions between calcium-binding proteins and TF families (WRKY, AP2/ERF) are vital for regulating downstream gene expression during the early osmotic phase of salt stress.
    • Identified SNPs offer insights into the molecular basis of differential gene expression and salt tolerance in wheat.