Related Experiment Video
Updated: Jun 8, 2025

Unraveling the Unseen Players in the Ocean - A Field Guide to Water Chemistry and Marine Microbiology
Published on: November 5, 2014
Intragenomic variations of the harmful algal bloom species Phaeocystis globosa through single-strain metabarcoding
1CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Ecology and Environmental Science, Qingdao Marine Science and Technology Center, Qingdao 266200, China; College of Marine Science, University of Chinese Academy of Sciences, Beijing 100039, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China.
Abstract:
Recent studies demonstrated that the existence of high-levels intragenomic variations (IGVs) may lead to overinterpretation of species diversity and genetic diversity in metabarcoding analysis. In this study, IGVs of the V4 region of 18S rRNA gene (18S rDNA V4) in Phaeocystis globosa were ascertained through metabarcoding analysis results of 54 P. globosa strains. Each P. globosa strain harbored over 200 ASVs, in which one ASV was dominant with higher relative abundance than others. According to different dominant ASVs, 54 strains could be divided into two groups, suggesting the high genetic diversity of P. globosa. ASVs were shared in different strains, suggesting that IGVs were genuine existed, rather than sequencing errors. Metabarcoding analysis of field samples identified large numbers of IGVs. High levels of IGVs may be due to incomplete homogenization or existence of nonfunctional pseudogenes. An accurate understanding of IGVs is necessary for the correct interpretation of metabarcoding analysis results.
More Related Videos
09:55Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere
Published on: May 2, 2018
08:09An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis
Published on: September 15, 2015
Related Concept Videos
Gene Evolution - Fast or Slow?
In contrast, regions which code...
Evolutionary Relationships through Genome Comparisons