The effect of Coix lachrymal L. seed extract on the expression of inflammation and fibrogenesis genes in human retinal pigment epithelial cells
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View abstract on PubMed
Summary
This summary is machine-generated.Adlay seed extract fractions show promise in treating proliferative vitreoretinopathy (PVR). The residual fraction, rich in bioactive compounds, effectively reduced PVR-associated pathways in retinal cells.
Area Of Science
- Ophthalmology
- Pharmacology
- Cell Biology
Background
- Proliferative vitreoretinopathy (PVR) is a major cause of vision loss following retinal detachment, driven by fibrocellular scar formation.
- Current treatments for PVR are limited, necessitating the exploration of novel therapeutic agents.
- Retinal pigment epithelial (RPE) cells play a critical role in PVR pathogenesis through proliferation, inflammation, and fibrogenesis.
Purpose Of The Study
- To investigate the therapeutic potential of adlay seed extract fractions (methanolic and residual) in mitigating PVR-associated pathways.
- To evaluate the effects of adlay fractions on oxidative stress, proliferation, inflammation, and fibrogenesis in RPE cells.
- To characterize the biochemical composition and antioxidant activity of adlay seed extract fractions.
Main Methods
- Adlay seed extract fractions (MeOH and Res) were prepared and analyzed for bioactive compounds and antioxidant capacity.
- RPE cell viability was assessed using the MTT assay after treatment with adlay fractions.
- Gene expression of key PVR-related markers (IL-1β, IL-6, LIF, TGF-β, Snail, α-SMA) was analyzed using real-time PCR.
Main Results
- The residual (Res) adlay fraction demonstrated higher protein, carbohydrate, flavonoid, and phenol content, along with superior antioxidant activity compared to the methanolic (MeOH) fraction.
- Both adlay fractions inhibited RPE cell viability, with the Res fraction showing a more significant effect.
- The MeOH fraction reduced IL-6 and TGF-β gene expression, while the Res fraction significantly decreased IL-6, LIF, TGF-β, Snail, and α-SMA expression.
Conclusions
- Adlay seed extract fractions possess therapeutic potential for PVR by modulating key pathogenic pathways in RPE cells.
- The Res fraction, due to its rich bioactive profile and potent antioxidant activity, is particularly promising for attenuating oxidative stress, proliferation, inflammation, and fibrogenesis.
- Adlay seed extracts represent a potential novel therapeutic strategy for PVR, warranting further preclinical and clinical investigation.
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