Multiomic analysis of Lewisite exposed human dermal equivalent tissues.

Area of Science:

• Toxicology
• Chemical Warfare Agents
• Dermatology

Background:

• Lewisite (L) is an arsenical vesicant chemical warfare agent (CWA) developed during WWI.
• Despite no documented use in warfare, significant global stockpiles remain.
• The potential for illicit production and existing stockpiles constitute a serious threat.

Purpose of the Study:

• To investigate the toxicological effects of Lewisite on human dermal equivalent tissues.
• To evaluate cellular viability, histological changes, and multiomic responses to Lewisite exposure.

Main Methods:

• Exposure of human dermal equivalent tissues (EpiDerm™/EpiDerm™-FT) to Lewisite.
• Assessment of cellular viability using standard assays.
• Histological examination of tissue damage.
• Comprehensive multiomic analysis to understand molecular-level responses.

Main Results:

• Lewisite exposure significantly impacted cellular viability in human dermal equivalent tissues.
• Histological analysis revealed characteristic tissue damage consistent with vesicant exposure.
• Multiomic analysis identified specific molecular pathways affected by Lewisite, providing insights into its mechanism of action.

Conclusions:

• Lewisite causes significant damage to human dermal equivalent tissues at cellular and histological levels.
• Multiomic analysis provides a deeper understanding of Lewisite's toxicological mechanisms.
• These findings highlight the continued threat posed by Lewisite and inform risk assessment and medical countermeasures.