Elastic and recoverable sponges based on collagen/yeast β-glucan for quick hemostasis

In this investigation, we aimed to engineer sponges with exceptional mechanical and hemostatic capabilities for effective wound healing. By combining collagen, a stiff fibril protein in ECM, with β-glucan, an elastic and triple-helical polysaccharide from yeast cell wall, we prepared a series of composite sponges, designated as CY sponges. This material exhibited a uniform pore structure, displaying enhanced elasticity and shape recovery ability compared to pure collagen sponges. Also, the incorporation of Yeast β-glucan (YG) significantly improved the fluid absorption ability and stability of the sponges. In vitro hemostasis tests demonstrated that the CY sponges exhibited a notably lower in vitro coagulation index (19.21 %) compared to the collagen control (64.84 %), accompanied by superior erythrocyte (64.64 %) and platelet (64.95 %) adhesion properties. Animal studies further substantiated the sponge's hemostatic efficacy, as CY40 led to a reduction in average bleeding volume by 25.26 % and 28.97 %, and a shorter hemostatic time by 31.70 % and 30.77 % compared to collagen, indicating accelerated wound healing. These findings suggest that the addition of yeast β-glucan into collagen sponges can improve their elasticity, shape recovery ability, hemostatic performance and wound repair ability.