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Evolutionary Relationships through Genome Comparisons

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Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...

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Related Experiment Video

Updated: Jun 8, 2025

Isolation and Transcriptome Analysis of Plant Cell Types

Isolation and Transcriptome Analysis of Plant Cell Types

Published on: April 7, 2023

Single same-cell multiome for dissecting key plant traits.

Rohini Garg¹, Sunil Kumar Sahu², Mukesh Jain³

¹Department of Life Sciences, School of Natural Sciences, Shiv Nadar Institution of Eminence, Gautam Buddha Nagar, Uttar Pradesh 201314, India; Centre of Excellence in Epigenetics, School of Natural Sciences, Shiv Nadar Institution of Eminence, Gautam Buddha Nagar, Uttar Pradesh 201314, India.

Trends in Plant Science

|November 1, 2024

Summary

This summary is machine-generated.

Single-cell multiome analysis reveals molecular dynamics underlying plant traits like stress response and development. These findings offer new insights into cellular pathways and regulatory networks.

Keywords:

chromatin accessibility development gene regulatory network single-cell transcriptome stress response

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Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

Published on: August 5, 2020

Area of Science:

Plant biology
Molecular biology
Genomics

Background:

Understanding molecular dynamics at the single-cell level is essential for deciphering complex plant traits.
Recent advancements in multiome analysis enable simultaneous measurement of multiple molecular layers within a single cell.

Purpose of the Study:

To investigate the molecular mechanisms of key plant traits, including osmotic stress response and pod development, at single-cell resolution.
To leverage multiome analysis to uncover novel insights into plant cellular pathways and regulatory networks.

Main Methods:

Multiome analysis (simultaneously analyzing multiple 'omes' like genomics, transcriptomics, etc.) was performed on single plant cells or nuclei.
Computational and bioinformatic approaches were used to dissect the data and identify key molecular players and interactions.

Main Results:

Liu et al. and Cui et al. successfully applied single-cell multiome analysis to study osmotic stress response and pod development.
The studies identified specific pathways and regulatory networks crucial for these plant traits at an unprecedented single-cell resolution.

Conclusions:

Single-cell multiome analysis is a powerful approach for understanding plant trait complexity.
These findings provide a foundation for future research into plant adaptation, development, and breeding strategies.