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Related Concept Videos

Supercritical Fluid Chromatography01:18

Supercritical Fluid Chromatography

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Supercritical fluid chromatography (SFC) provides a beneficial substitute for gas chromatography (GC) and liquid chromatography (LC) for certain samples because it merges the top attributes of both techniques. SFC allows the separation and analysis of compounds that GC or LC does not easily manage. These compounds are traditionally nonvolatile or thermally unstable, making GC unsuitable and lacking functional groups required for HPLC analysis.
SFC utilizes a supercritical fluid mobile phase,...
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High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
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High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

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High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
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Principles Of Column Chromatography01:13

Principles Of Column Chromatography

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The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
6.7K
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

429
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
429
Affinity Chromatography01:03

Affinity Chromatography

543
Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
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Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
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Supercritical Fluid Chromatography in Bioanalysis-A Review.

Laura Toribio1, María Teresa Martín1, José Bernal1

  • 1Department of Analytical Chemistry, Faculty of Sciences, I. U. CINQUIMA, Analytical Chemistry Group (TESEA), University of Valladolid, Valladolid, Spain.

Journal of Separation Science
|November 2, 2024
PubMed
Summary
This summary is machine-generated.

Supercritical fluid chromatography (SFC) coupled with mass spectrometry (MS) is a powerful analytical tool. Recent advancements have expanded its use in bioanalysis due to unique selectivity and greener methods.

Keywords:
Metabolomicsdoping analysisforensic analysislipidomics

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Area of Science:

  • Analytical Chemistry
  • Bioanalysis

Background:

  • Supercritical fluid chromatography (SFC) has seen significant improvements in instrumentation and hyphenation with mass spectrometry (MS) over the last decade.
  • These advancements have led to increased acceptance and application of SFC in analytical chemistry, particularly in bioanalysis.

Purpose of the Study:

  • To review the advantages and main applications of SFC in bioanalysis over the past 5 years.
  • To discuss fundamental aspects including mobile phase composition, stationary phase selection, MS hyphenation, and matrix effects.

Main Methods:

  • Review of recent literature on SFC applications in bioanalysis.
  • Discussion of key technical considerations for SFC-MS in biological sample analysis.

Main Results:

  • SFC offers unique selectivity, rapid analysis times, reduced organic solvent consumption, and a greener mobile phase.
  • Increased publications highlight SFC's growing role in bioanalysis.

Conclusions:

  • SFC is a valuable and increasingly utilized tool in bioanalysis.
  • Understanding mobile phase, stationary phase, MS hyphenation, and matrix effects is crucial for successful SFC applications in bioanalysis.