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Updated: Jun 8, 2025
Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
Published on: July 3, 2018
Circulating microRNA-409-5p and USP7 are associated with left ventricular remodeling in patients with acute
Guiping He1,2, Sha Sha3, Jiaji He1,2
1Department of Cardiology, Yan'an Hospital of Kunming City, Yan'an Hospital Affiliated To Kunming Medical University, Kunming, 650051, China.
Background:
Our previous study demonstrated that microRNA-409-5p (miR-409-5p) and its target ubiquitin-specific protease 7 (USP7) were involved in hypoxia-induced cardiomyocyte injury and ischemic left ventricular remodeling (LVR) in rats. This study aimed to probe into the relationship between plasma miR-409-5p and USP7 levels and LVR and dysfunction in patients with acute myocardial infarction (AMI).
Methods:
Sixty patients with acute myocardial infarction (AMI) and 60 cases with chronic coronary syndrome (CCS) were enrolled. The clinical characteristics, echocardiographic/serum parameters of LVR, and circulating miR-409-5p and USP7 mRNA levels between the two groups and between admission and 6 weeks after discharge were compared. The correlations between circulating miR-409-5p/USP7 levels and serum/echocardiographic parameters were analyzed.
Results:
The demographic characteristics of the AMI group and CCS group were comparable. Patients with AMI admitted to the study displayed significantly higher levels of plasma N-terminal pro-brain natriuretic peptide (NT-proBNP) and growth stimulation expressed gene 2 (ST2), along with a greater left ventricular end-systolic volume (LVESV). Conversely, their left ventricular ejection fraction (LVEF), global longitudinal strain (GLS), and global radial strain (GRS) were significantly lower compared to patients with CCS. These changes were normalized 6 weeks after discharge. Circulating miR-409-5p levels at admission were significantly decreased while circulating USP7 mRNA expression levels were significantly increased in patients with AMI compared with those with CCS (both P < 0.01). However, these changes were restored 6 weeks after discharge (both P < 0.01). Moreover, circulating miR-409-5p and USP7 mRNA levels showed varying correlations with GLS, GRS, LVESV, LVEF, and NT-proBNP in patients with AMI but not in those with CCS. Additionally, circulating miR-409-5p and USP7 levels predicted the incidence of LVR and major adverse cardiovascular events (MACE) after AMI.
Conclusion:
Serum miR-409-5p and USP7 may influence the occurrence and evolution of LVR and left ventricular dysfunction after AMI.
Insights
Plasma levels of microRNA-409-5p (miR-409-5p) and ubiquitin-specific protease 7 (USP7) are linked to left ventricular remodeling (LVR) and dysfunction in acute myocardial infarction (AMI) patients. These markers normalized post-discharge, predicting LVR and adverse events.
Area of Science:
- Cardiovascular Biology
- Molecular Cardiology
- Biomarker Discovery
Background:
- Previous research linked microRNA-409-5p (miR-409-5p) and ubiquitin-specific protease 7 (USP7) to hypoxia-induced cardiomyocyte injury and left ventricular remodeling (LVR) in rats.
- The study investigates the association between plasma miR-409-5p and USP7 levels with LVR and dysfunction in patients experiencing acute myocardial infarction (AMI).
Purpose of the Study:
- To determine the relationship between circulating miR-409-5p and USP7 levels and left ventricular remodeling (LVR) and dysfunction in patients with acute myocardial infarction (AMI).
- To assess the predictive value of these biomarkers for LVR and major adverse cardiovascular events (MACE) post-AMI.
Main Methods:
- Compared clinical characteristics, echocardiographic/serum parameters of LVR, and circulating miR-409-5p and USP7 mRNA levels in 60 AMI patients versus 60 chronic coronary syndrome (CCS) patients.
- Analyzed correlations between biomarker levels and LVR/dysfunction parameters at admission and 6 weeks post-discharge.
Main Results:
- AMI patients exhibited significantly higher plasma N-terminal pro-brain natriuretic peptide (NT-proBNP) and ST2, greater left ventricular end-systolic volume (LVESV), and lower left ventricular ejection fraction (LVEF), global longitudinal strain (GLS), and global radial strain (GRS) compared to CCS patients.
- Circulating miR-409-5p was decreased while USP7 mRNA was increased in AMI patients at admission, with normalization 6 weeks post-discharge.
- miR-409-5p and USP7 levels correlated with GLS, GRS, LVESV, LVEF, and NT-proBNP in AMI patients and predicted LVR and MACE incidence.
Conclusions:
- Plasma miR-409-5p and USP7 levels are significantly altered in patients with AMI and are associated with left ventricular remodeling and dysfunction.
- These biomarkers may play a role in the development and progression of LVR and left ventricular dysfunction following AMI.
- Circulating miR-409-5p and USP7 levels show potential as predictors for LVR and major adverse cardiovascular events after AMI.
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