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Updated: Jun 12, 2025
Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions
Published on: October 21, 2016
Protocol for evaluating drug-protein interactions based on fluorescence spectroscopy
Elaheh Jalali1, Javad Sargolzaei1
1Department of Biology, Faculty of Science, Arak University, Arak, Iran.
Abstract:
Investigating the interactions between ligands, such as drugs, and proteins is essential to understand their functions and effects. This fluorescence spectroscopy protocol details how to analyze the effects of drugs on protein structure, including steps for solution preparation, fluorescence spectra collection, and results analysis. It is applicable to studying drug-protein interactions, binding affinity, and thermodynamic parameters as well as performing further studies on drug design. For complete details on the use and execution of this protocol, please refer to Jalali et al.1 and Sargolzaei et al.2.
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