Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Decoding the epithelial-stromal interactome in allergic rhinitis through single-cell multi-omics integration.

The Journal of allergy and clinical immunology·2026
Same author

Metabolic dysfunction-associated fatty liver disease in chronic hepatitis B: dual effects on hepatocarcinogenesis and evolving strategies for risk prediction.

Frontiers in immunology·2026
Same author

Burden of Malaria and Dengue Across Global, Asian, and Chinese Populations Based on GBD 2021 Data: A Quantitative Assessment of Importation Risks to China.

Viruses·2026
Same author

Variations in rice yield and quality during the early and late seasons under the HLREP-CC model across different ecological zones.

Journal of the science of food and agriculture·2026
Same author

Clinical research progress and challenges of vaccine for human papillomavirus-associated cancers.

Human vaccines & immunotherapeutics·2026
Same author

Envisioning population-scale immune multi-omics atlas projects.

Clinical and translational medicine·2026

Related Experiment Video

Updated: Jun 12, 2025

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

6.6K

A protocol for acquiring high-quality single-cell multi-omics data from human peripheral blood.

Shanshan Duan1, Guokang Ma2, Junjie Chen2

  • 1BGI Research, Hangzhou 310030, China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.

STAR Protocols
|November 3, 2024
PubMed
Summary

This study details a new protocol for high-quality single-cell multi-omics analysis of human peripheral blood mononuclear cells (PBMCs). The method ensures data quality and cell viability for immune system research.

Keywords:
Biotechnology and bioengineeringClinical ProtocolGenomics

More Related Videos

Author Spotlight: Advancing Biomedical Research Through Single Cell Analysis
07:59

Author Spotlight: Advancing Biomedical Research Through Single Cell Analysis

Published on: December 22, 2023

2.5K
Author Spotlight: Advancing In Vitro Blood Cell Production with Single-Cell Multiomics and Functional Genomics
04:11

Author Spotlight: Advancing In Vitro Blood Cell Production with Single-Cell Multiomics and Functional Genomics

Published on: March 15, 2024

1.0K

Related Experiment Videos

Last Updated: Jun 12, 2025

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

6.6K
Author Spotlight: Advancing Biomedical Research Through Single Cell Analysis
07:59

Author Spotlight: Advancing Biomedical Research Through Single Cell Analysis

Published on: December 22, 2023

2.5K
Author Spotlight: Advancing In Vitro Blood Cell Production with Single-Cell Multiomics and Functional Genomics
04:11

Author Spotlight: Advancing In Vitro Blood Cell Production with Single-Cell Multiomics and Functional Genomics

Published on: March 15, 2024

1.0K

Area of Science:

  • Immunology
  • Genomics
  • Proteomics
  • Metabolomics

Background:

  • Single-cell analysis offers deep insights into immune system function.
  • Maintaining cell viability and data quality is crucial for accurate single-cell sequencing.
  • Existing protocols may require optimization for comprehensive multi-omics analysis.

Purpose of the Study:

  • To present a robust protocol for high-quality single-cell multi-omics data acquisition from human PBMCs.
  • To enable simultaneous analysis of genomic, proteomic, and metabolomic profiles at the single-cell level.
  • To improve the reliability and depth of insights from peripheral blood immune cell studies.

Main Methods:

  • Development of a modified multi-omics sample processing workflow.
  • Detailed steps for human blood collection and PBMC isolation.
  • Integration of single-cell sequencing, whole-genome sequencing, and metabolome/proteome analysis.

Main Results:

  • Successful acquisition of high-quality single-cell multi-omics data.
  • Demonstration of protocol's efficacy in preserving cell viability.
  • Generation of comprehensive datasets for immune cell profiling.

Conclusions:

  • The presented protocol is effective for high-throughput single-cell multi-omics analysis of PBMCs.
  • This method enhances the understanding of innate and adaptive immunity.
  • The protocol serves as a valuable tool for immunological research and biomarker discovery.