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Related Experiment Video

Updated: Jun 8, 2025

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

Heat application in live cell imaging.

Linda Sistemich^1,2, Simon Ebbinghaus^1,2

¹Chair of Biophysical Chemistry, Ruhr-University Bochum, Germany.

|November 3, 2024

Summary

This summary is machine-generated.

Precise thermal heating and temperature sensing are crucial for manipulating cellular processes in live-cell imaging. This review covers various heating techniques and their challenges for biological applications.

Keywords:

fluorescence microscopy live‐cell imaging microheating thermometry

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Last Updated: Jun 8, 2025

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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

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Published on: December 9, 2013

Area of Science:

Cell Biology
Biophysics
Live-cell Imaging

Background:

Thermal heating offers precise control over cellular processes.
Live-cell imaging requires accurate temperature monitoring for reliable results.

Purpose of the Study:

To review current thermal heating and temperature sensing techniques for live-cell imaging.
To discuss challenges and future applications in cell biology.

Main Methods:

Peltier elements and microfluidics for homogenous heating.
Infrared lasers and nanostructure-based absorption for spot heating.
Precise temperature measurement techniques for biological samples.

Main Results:

Various heating methods exist, each with specific applications.
Accurate temperature sensing remains a key challenge, especially at subcellular levels.
The integration of heating and sensing is vital for controlled cellular manipulation.

Conclusions:

Advancements in thermal control are essential for live-cell imaging.
Future research should focus on improving temperature measurement accuracy.
These techniques hold promise for novel cell biology applications.