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Polyhedrin microcrystals embedded with bFGF promote wound healing
Mei Yin1, Xinyu Tong1, Yongjie Feng1
1School of Life Science, Soochow University, Suzhou 215123, China.
International Journal of Biological Macromolecules
|November 3, 2024
Summary
This study developed a novel method to embed basic fibroblast growth factor (bFGF) into Bombyx mori cypovirus (BmCPV) polyhedra for sustained release. These bFGF-polyhedra significantly accelerated wound healing in animal models by promoting cell growth and regeneration.
Area of Science:
- Biotechnology
- Wound Healing Research
- Insect Virology
Background:
- Growth factors are crucial for wound healing, necessitating effective sustained-release systems.
- Bombyx mori cypovirus (BmCPV) produces polyhedra that encapsulate viral particles.
- Viral structural protein VP7 interacts with polyhedrin (Polh), forming the polyhedra structure.
Purpose of the Study:
- To investigate the potential of BmCPV polyhedra as a biosustained-release system for growth factors.
- To develop a method for incorporating basic fibroblast growth factor (bFGF) into BmCPV polyhedra.
- To evaluate the efficacy of bFGF-loaded polyhedra in promoting wound healing.
Main Methods:
- Identified the VP7-tag (amino acids 331-360) of VP7 as essential for Polh interaction.
- Utilized a baculovirus expression system to co-express Polh and a bFGF-VP7 fusion protein.
- Constructed bFGF-polyhedra and assessed bFGF-VP7 release, stability, and biological activity in vitro and in vivo.
Main Results:
- VP7-tag facilitated the incorporation of bFGF-VP7 into polyhedra.
- bFGF-polyhedra exhibited sustained release of bFGF-VP7, enhancing cell proliferation and migration.
- In vivo studies showed bFGF-polyhedra significantly improved full-thickness wound healing by modulating key molecular pathways and promoting tissue regeneration.
Conclusions:
- BmCPV polyhedra serve as an effective biosustained-release system for bFGF.
- bFGF-polyhedra demonstrate significant potential for accelerating wound healing through enhanced cell proliferation, differentiation, and tissue regeneration.
- This approach offers a promising strategy for developing advanced wound healing therapeutics.
