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  1. Home
  3. Black Carp Rnf135 Enhances Rig-i-mediated Antiviral Signaling By Facilitating Its Oligomerization.
  1. Home
  3. Black Carp Rnf135 Enhances Rig-i-mediated Antiviral Signaling By Facilitating Its Oligomerization.

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Black carp RNF135 enhances RIG-I-mediated antiviral signaling by facilitating its oligomerization.

Chushan Dai1, Yujia Miao1, Zhan'ao Li1

  • 1State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, 410081, China.

Fish & Shellfish Immunology
|November 4, 2024

View abstract on PubMed

Summary
This summary is machine-generated.

Black carp RNF135 (bcRNF135) enhances antiviral immunity by boosting RIG-I signaling. This protein facilitates RIG-I activation, crucial for teleost innate immune responses against viral infections.

Keywords:
Black carpIFNOligomerizationRIG-IRNF135

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Area of Science:

  • Immunology
  • Molecular Biology
  • Fish Biology

Background:

  • RNF135 (RIPLET) is vital for RIG-I signaling in mammals.
  • The role and regulation of RNF135 in teleosts are not well understood.

Purpose of the Study:

  • To clone and characterize the black carp RNF135 homolog (bcRNF135).
  • To investigate the function of bcRNF135 in teleost antiviral immunity, particularly its interaction with bcRIG-I.

Main Methods:

  • Gene cloning and sequencing of bcRNF135.
  • Protein characterization using immunoblotting (IB) and immunofluorescence (IF).
  • Gene expression analysis via qRT-PCR, reporter assays, co-immunoprecipitation (Co-IP), and native PAGE (SDD-AGE).

Main Results:

  • bcRNF135 is a 50 kDa cytoplasmic protein that enhances bcRIG-I-mediated IFN transcription and antiviral responses.
  • bcRNF135 expression is upregulated upon viral infection (SVCV) and poly(I:C) stimulation.
  • Knockdown of bcRNF135 impairs host antiviral defense and promotes viral replication.
  • bcRNF135 interacts with bcRIG-I and promotes its oligomerization, a key step in RIG-I activation.

Conclusions:

  • bcRNF135 significantly enhances RIG-I-mediated antiviral signaling in black carp.
  • The mechanism involves facilitating bcRIG-I ubiquitination and oligomerization.
  • This study deepens the understanding of RIG-I pathway regulation in teleost innate immunity.