Injectable Nanoengineered Adhesive Hydrogel for Treating Enterocutaneous Fistulas

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Gastroenterology

Background:

• Enterocutaneous fistula (ECF) is a severe complication, often arising from surgery, leading to high morbidity and mortality.
• Current treatments for ECF face challenges, necessitating innovative solutions for effective sealing and healing.
• The constant leakage from ECF causes significant skin breakdown and infection risks.

Purpose of the Study:

• To develop and evaluate an injectable nanoengineered adhesive hydrogel (INAH) for the treatment of ECF.
• To assess the mechanical, adhesive, and biocompatible properties of the novel hydrogel.
• To investigate the potential of INAH in promoting tissue regeneration and healing of ECF.

Main Methods:

• Development of an injectable nanoengineered adhesive hydrogel (INAH) using Laponite® and a gelatin-dopamine conjugate.
• Co-injection method for fast cross-linking, enhancing mechanical and adhesive properties.
• Ex vivo and in vivo assessments of hydrogel biocompatibility, adhesion, blood clotting, and tissue integration.

Main Results:

• The INAH exhibited rapid cross-linking, improved mechanical strength, and strong adhesive properties in hydrated environments.
• Demonstrated effective blood clotting ability and cytocompatibility with fibroblasts.
• In vivo studies confirmed INAH biocompatibility, supporting cell infiltration and extracellular matrix deposition without fibrosis.

Conclusions:

• The developed INAH presents a promising injectable solution for sealing and healing enterocutaneous fistulas.
• Its properties facilitate tissue integration and regeneration, offering potential for ECF treatment.
• INAH holds significant translational potential in regenerative medicine and tissue engineering for complex wound healing.