Probiotic-Derived P8 Protein: Promoting Proliferation and Migration in Stem Cells and Keratinocytes

Area of Science:

• Microbiology
• Cell Biology
• Biochemistry

Background:

• Probiotics offer health benefits, with Lactobacillus rhamnosus P8 protein showing anticancer properties.
• The effects of P8 protein on stem cells and normal cells, particularly in wound healing, remain largely unexplored.

Purpose of the Study:

• To investigate the impact of P8 protein on cell proliferation and wound healing.
• To elucidate the underlying molecular mechanisms of P8 protein's effects on stem cells and keratinocytes.

Main Methods:

• Scratch assays were performed to evaluate wound healing.
• Stem cells were cultured with P8 protein to assess proliferation, migration, and stemness marker expression.
• Key signaling pathways, including MAPK and Hippo, were analyzed.

Main Results:

• P8 protein significantly accelerated wound healing by activating the cell cycle and promoting stem cell stemness.
• P8 enhanced stem cell proliferation and migration, upregulating OCT4 and SOX2 expression.
• Activation of MAPK and Hippo signaling pathways was observed, contributing to enhanced cell growth and migration.

Conclusions:

• P8 protein promotes cell growth, migration, and stemness via MAPK and Hippo signaling, leading to improved wound healing.
• P8 protein enhances keratinocyte wound healing by activating MAPK signaling pathways.
• P8 protein shows potential for improving stem cell culture efficiency and treating skin diseases.