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Development of a MALDI-TOF MS model for differentiating haemorrhagic septicaemia-causing strains of Pasteurella

Kelli Maddock1, Brianna L S Stenger2, Jill C Roberts3

  • 1Veterinary Diagnostic Laboratory, North Dakota State University, Fargo, ND, USA; College of Public Health, University of South Florida, Tampa, FL, USA.

Journal of Microbiological Methods
|November 4, 2024
PubMed

Insights

A new matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) method accurately distinguishes haemorrhagic septicaemia strains of Pasteurella multocida. This rapid diagnostic tool can aid in identifying severe P. multocida infections in animals.

Area of Science:

  • Veterinary Microbiology
  • Animal Infectious Diseases
  • Mass Spectrometry Applications

Background:

  • Pasteurella multocida causes significant animal diseases, including bovine respiratory disease and devastating haemorrhagic septicaemia.
  • Current diagnosis of haemorrhagic septicaemia relies on lesion recognition and laborious serotyping or molecular methods.
  • Haemorrhagic septicaemia, caused by P. multocida capsular types B and E, poses a high mortality risk and has pandemic potential.

Purpose of the Study:

  • To develop and validate a rapid diagnostic method for differentiating haemorrhagic septicaemia-causing P. multocida strains from other types.
  • To utilize genomic data for creating a biomarker-based assay for P. multocida strain identification.
  • To improve the timely diagnosis of haemorrhagic septicaemia for effective disease management.

Main Methods:

  • Genomic characterization of 84 P. multocida strains, including haemorrhagic septicaemia types (B:2,5, E:2,5, B:3,4).
  • Development of a matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) method using specific protein peaks (6419 and 7729 m/z).
  • Validation of three automated classification models and an assisted model for strain differentiation.

Main Results:

  • The assisted MALDI-TOF MS model achieved high accuracy: 98.2% for non-haemorrhagic septicaemia strains and 100% for classic haemorrhagic septicaemia strains (B:2,5 and E:2,5).
  • Overall accuracy for differentiating haemorrhagic septicaemia strains (B:2,5, E:2,5, B:3,4) was 96.9%.
  • The method demonstrated robust performance in distinguishing between disease-causing P. multocida types.

Conclusions:

  • MALDI-TOF MS offers a rapid and accurate approach for the initial screening of P. multocida isolates.
  • This method can facilitate the early identification of haemorrhagic septicaemia-causing strains in diagnostic settings.
  • Routine implementation of this MALDI-TOF MS assay can guide further characterization and disease control strategies.
Keywords:
Bovine respiratory disease complexHaemorrhagic septicaemiaMALDI MSPasteurella multocidaPasteurellosis

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