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Published on: May 2, 2018
Differential expression of six cytokines in Rattus rattus exposed to leptospirosis: A comprehensive transcriptomic
Nurul Huda Mohamad Ikbal1, Subha Bhassu2, Lay Kek Teh3
1Integrative Pharmacogenomics Institute (iPROMISE), Faculty of Pharmacy, Universiti Teknologi MARA Puncak Alam Campus, 42300 Puncak Alam, Selangor, Malaysia; Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia; Centre for Research in Biotechnology for Agriculture (CEBAR), Research Management and Innovation Complex, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Background:
Rattus rattus are the main carriers of various zoonotic diseases including leptospirosis. Regrettably, information underlying the cytokine response of wild R. rattus upon leptospirosis infection is lacking. This study aims to understand the immune response presented by specifically the kidney and liver of R. rattus during leptospirosis infection.
Methodology:
High-throughput RNA-Sequencing technology was employed to discover the transcriptome alterations in the kidney and liver of R. rattus during natural infection. Both kidney and liver tissues from the healthy and infected rats were sequenced using the BGISEQ-500 sequencing platform. The GO and KEGG databases were utilized to functionally annotate the differentially expressed transcripts of the selected cytokines; TNF-α, IL-1β, IL-6, IL-10, MIP-1α, and IFN-γ.
Results:
A higher number of upregulated genes were signified in the kidney as compared to the liver during infection. Among the six selected cytokines, Interleukin-6 was found to be expressed during the early stage in the liver of R. rattus, while all the other six genes were upregulated during the late stage of leptospirosis in the kidney of R. rattus. The GO of the annotated genes was classified under inflammatory response and cellular response to lipopolysaccharide, while the KEGG pathway indicated cytokine-cytokine receptor interaction and the Toll-like receptor (TLR) signalling pathway. The upshots of this study correlated the different phases of cytokine response in different organs of R. rattus during leptospirosis infection.
Conclusion:
Overall, these studies formulate a conceptual framework based on host and pathogen relationships of leptospirosis transmission patterns and the discovery of biomarkers in tracking the early stages of leptospires colonization.
