An ecological explanation for carbon source-associated denitrification performance in wastewater treatment plants

The underlying mechanism associated with the roles of dosed carbon source in denitrification performance remains largely unknown. In this study, three denitrifying consortia (DNC) were constructed via evolutionary top-down enrichment method with well-defined conditions and specific carbon sources (acetate, glucose and their mixture). The reactor operation shows that nearly complete nitrate removal was achieved; however, the glucose feeding resulted in much higher concentrations of biomass and non-settable flocs. The 16S rRNA sequencing suggests that the bacterial diversity of the acetate-fed DNC was significantly higher than those of acetate/glucose-fed and glucose-fed DNCs. The dentrifying population in the acetate-fed DNC was dominated by Propionivibrio (16.1 %) and Thauera (3.4 %); whereas those of acetate/glucose- and glucose-fed DNCs were dominated by Pleomorphomonas (21.5 % and 26.3 %, respectively). Interestingly, the supernatant of acetate-fed DNC contained a high abundance of genera Thauera (averaged at 85.1 %), indicating the free-living nature of Thauera. Both PICURSt2 analysis of 16S rRNA sequencing and metagenomic analysis indicate that the acetate-fed DNC contained higher abundances of denitrifying genes; the acetate/glucose-fed and glucose-fed DNCs, in comparison, enriched genes related to glucose transportation and metabolism. Additionally, the acetate-fed DNC had better network stability than other two groups. This study adds important knowledge regarding the ecological traits of DNC, providing important clues for rational addition of carbon sources in wastewater treatment plants.