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Advanced LC-MS/MS Technique for Environmental Ivermectin Detection.

Alicia Maria Carrillo Heredero1, Giulia Segato2, Simonetta Menotta2

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Summary
This summary is machine-generated.

A new, validated method accurately detects ivermectin in environmental samples like feces, soil, and sewage. This sensitive assay is crucial for monitoring ivermectin

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Area of Science:

  • Environmental Chemistry
  • Analytical Chemistry
  • Ecotoxicology

Background:

  • Ivermectin, a broad-spectrum antiparasitic, poses toxicity risks to non-target aquatic and insect species.
  • Existing detection methods lack the required accuracy and sensitivity for comprehensive environmental monitoring.
  • Understanding ivermectin's environmental fate necessitates precise quantification in diverse matrices.

Purpose of the Study:

  • To develop and validate a highly accurate analytical method for ivermectin detection.
  • To assess ivermectin levels in environmental samples including feces, soil, and sewage.
  • To establish a sensitive assay for environmental risk assessment and monitoring.

Main Methods:

  • Method development and validation following Eurachem guidelines.
  • Quantification of ivermectin in feces, soil, and sewage matrices.
  • Determination of method's limit of detection (LOD) and limit of quantification (LOQ).

Main Results:

  • The validated method demonstrated high linearity (R² ≥ 0.99) and recovery rates (92-96%).
  • Low limits of detection were achieved: 0.66 μg/kg (feces), 0.54 μg/kg (soil), and 0.36 μg/kg (sewage).
  • The method was successfully applied to analyze 333 environmental samples.

Conclusions:

  • A precise and sensitive method for ivermectin detection in environmental matrices was successfully developed and validated.
  • This assay enables accurate ivermectin quantification, supporting environmental monitoring and risk assessment.
  • The method's robustness and sensitivity offer improved capabilities for ecotoxicological studies.