Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fixation and Sectioning01:03

Fixation and Sectioning

4.2K
Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
4.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Untargeted Metabolomics Analysis Reveals Dynamic Shift in Follicular Fluid Metabolites After GnRH Induced Ovulation in Bos taurus Cattle.

Molecular reproduction and development·2026
Same author

Inhibiting Bacterioferritin Iron Release Induces Iron Starvation and Metabolic Downshift, Potentiating Aminoglycosides in <i>P. aeruginosa</i> Biofilms.

ACS infectious diseases·2026
Same author

Integrative Raman Spectroscopy and Multi-Omics Analysis of Lipid and Matrix Protein Heterogeneity in Triple-Negative Breast Cancer.

Chemical & biomedical imaging·2026
Same author

Unveiling ribosomal dysfunction and impaired signaling pathways in the cortical region of 5xFAD mice.

Acta neuropathologica communications·2026
Same author

Deep Ultraviolet Laser Ablation Electrospray Ion Mobility Mass Spectrometry.

Journal of the American Society for Mass Spectrometry·2026
Same author

Systemic Accumulation and Deterrent Effects of Ipomeamarone in Sweetpotato Weevil-Injured Storage Roots.

ACS agricultural science & technology·2025

Related Experiment Video

Updated: Jun 8, 2025

Protocol for HER2 FISH Using a Non-cross-linking, Formalin-free Tissue Fixative to Combine Advantages of Cryo-preservation and Formalin Fixation
10:21

Protocol for HER2 FISH Using a Non-cross-linking, Formalin-free Tissue Fixative to Combine Advantages of Cryo-preservation and Formalin Fixation

Published on: December 25, 2017

13.6K

Infrared Laser Ablation and Capture of Formalin-Fixed Paraffin-Embedded Tissue.

Blessing C Egbejiogu1, Fabrizio Donnarumma1, Kermit K Murray1

  • 1Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana 70803, United States.

Journal of the American Society for Mass Spectrometry
|November 4, 2024
PubMed
Summary

Laser ablation sampling effectively analyzes proteins in formalin-fixed paraffin-embedded (FFPE) tissues, identifying more proteins than other methods. This technique overcomes challenges in FFPE tissue proteomics, enabling better research and clinical applications.

Keywords:
formalin-fixed paraffin-embedded tissuefresh frozen tissuelaser ablationmanual dissectionproteomics

More Related Videos

High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
11:05

High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

Published on: January 21, 2015

33.2K
Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue
09:20

Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue

Published on: September 2, 2013

27.6K

Related Experiment Videos

Last Updated: Jun 8, 2025

Protocol for HER2 FISH Using a Non-cross-linking, Formalin-free Tissue Fixative to Combine Advantages of Cryo-preservation and Formalin Fixation
10:21

Protocol for HER2 FISH Using a Non-cross-linking, Formalin-free Tissue Fixative to Combine Advantages of Cryo-preservation and Formalin Fixation

Published on: December 25, 2017

13.6K
High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
11:05

High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

Published on: January 21, 2015

33.2K
Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue
09:20

Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue

Published on: September 2, 2013

27.6K

Area of Science:

  • Biomedical research
  • Proteomics
  • Analytical chemistry

Background:

  • Formalin-fixed paraffin-embedded (FFPE) tissues are crucial for research but challenging for proteomics due to protein modifications.
  • Existing methods for FFPE tissue analysis are limited by protein cross-linking and chemical alterations.

Purpose of the Study:

  • To evaluate laser ablation sampling for precise microsampling of FFPE rat liver tissue for proteomics.
  • To compare the efficiency of laser ablation sampling with other methods for FFPE tissue protein analysis.

Main Methods:

  • Pulsed mid-infrared laser microsampling was used to extract material from FFPE rat liver tissue.
  • Proteins were identified and quantified using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS).
  • Comparisons were made between FFPE tissue laser ablation, fresh-frozen (FF) tissue laser ablation, and manual dissection of FFPE tissue.

Main Results:

  • Laser ablation sampling identified more proteins from FFPE tissue than from FF tissue.
  • More proteins were identified by laser ablation from FFPE tissue compared to manual dissection.
  • No loss of hydrophilic proteins was observed, suggesting efficient protein capture and minimal residual cross-linking effects.

Conclusions:

  • Laser ablation sampling is a highly effective method for FFPE tissue proteomics, surpassing traditional techniques.
  • The laser's ability to break up tissue facilitates downstream protein processing and analysis.
  • This technique enhances the utility of FFPE tissues for biomedical research and clinical applications.