Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A cocktail of human mAbs targeting the henipavirus fusion and receptor binding proteins provides cross-species neutralization.

Science translational medicine·2026
Same author

Harms of selected spinal and paraspinal injections and denervation procedures for chronic non-cancer spine pain: a systematic review and meta-analysis of non-randomised studies.

BMJ open·2026
Same author

Epigenetic control of telomeric RNA maintains heterochromatin in telomerase-driven cancers.

Signal transduction and targeted therapy·2026
Same author

In Vitro Antiviral Properties of Two Recombinant Sendai Virus Vectors Encoding <i>ORFV 011</i> and <i>ORFV 059</i> Genes.

Viruses·2026
Same author

HIV Nef-mediated WAVE2-ARP2/3 inhibition underlies CD4<sup>+</sup> T-cell lamellipodial abnormalities and immune dysfunction.

mBio·2026
Same author

Knockdown of the long isoform of the prolactin receptor selectively targets pathogenic immune cells in systemic lupus erythematosus and averts glomerular pathology.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Jun 15, 2026

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors
09:16

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors

Published on: October 30, 2016

11.2K

A temperature-sensitive and less immunogenic Sendai virus for efficient gene editing.

Christian S Stevens1, Jillian C Carmichael1, Ruth Watkinson1

  • 1Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.

Journal of Virology
|November 4, 2024
PubMed
Summary

A novel temperature-sensitive Sendai virus (ts SeV) effectively delivers CRISPR-Cas9 gene editing tools. This safe and efficient delivery method shows promise for treating genetic disorders and HIV infection.

Keywords:
CCR5CRISPR/Cas9HIVParamyxoviridaeSendai virusgene editinghematopoietic stem and progenitor cellsmonocytesviral vector

More Related Videos

A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells
10:42

A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells

Published on: December 12, 2017

15.1K
Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing
12:21

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

Published on: January 7, 2019

13.2K

Related Experiment Videos

Last Updated: Jun 15, 2026

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors
09:16

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors

Published on: October 30, 2016

11.2K
A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells
10:42

A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells

Published on: December 12, 2017

15.1K
Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing
12:21

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

Published on: January 7, 2019

13.2K

Area of Science:

  • Gene editing and viral vector development for therapeutic applications.

Background:

  • Gene editing holds promise for treating genetic diseases like HIV, beta-thalassemia, and sickle cell disease.
  • Current gene editing therapies face limitations due to risks associated with DNA viral vectors.
  • An alternative delivery strategy is needed to overcome these limitations and advance gene editing therapies.

Purpose of the Study:

  • To develop a novel, safe, and effective delivery vector for CRISPR-Cas9 gene editing.
  • To utilize a temperature-sensitive Sendai virus (ts SeV) for efficient gene editing in sensitive human cell types.
  • To assess the potential of ts SeV in reducing innate immune responses and enabling personalized medicine.

Main Methods:

  • Development of a temperature-sensitive and less immunogenic Sendai virus (ts SeV) as a delivery vector.
  • Utilized ts SeV for delivering CRISPR-Cas9 in primary human CD14+ monocytes and CD34+ hematopoietic stem and progenitor cells (HSPCs).
  • Evaluated transduction efficiency in HSPC subpopulations and measured CCR5 editing frequency and HIV-1 inhibition.

Main Results:

  • ts SeV demonstrated high transduction efficiency in human CD34+ HSPCs, including stem cell-enriched subpopulations.
  • Achieved over 90% CCR5 editing frequency and over 70% bi-allelic CCR5 editing in primary human CD14+ monocytes.
  • Demonstrated significant inhibition of HIV-1 infection in primary human CD14+ monocytes ex vivo.

Conclusions:

  • The ts SeV platform is a safe, efficient, and flexible tool for delivering CRISPR-Cas9 gene editing machinery.
  • This novel delivery method minimizes innate immune responses, enhancing its therapeutic potential.
  • ts SeV shows promise for expanding personalized medicine and treating genetic disorders and infectious diseases like HIV.