MicroRNA-181b-5p/HEY2 axis is involved in the progress of deep venous thrombosis via mediating vascular endothelial...

Area of Science:

• Vascular Biology
• Molecular Medicine
• Biomarker Discovery

Background:

• Deep-venous thrombosis (DVT) is a serious condition with post-thrombotic syndrome (PTS) as a frequent complication.
• Understanding the molecular mechanisms underlying DVT progression is crucial for developing effective diagnostic and therapeutic strategies.

Purpose of the Study:

• To investigate the role of microRNA 181b-5p (miR-181b-5p) in the development and progression of DVT.
• To assess the diagnostic potential of miR-181b-5p for DVT and its association with PTS.
• To elucidate the cellular mechanisms by which miR-181b-5p influences endothelial cell function.

Main Methods:

• Quantification of miR-181b-5p levels in 150 patients with acute lower extremity DVT.
• Analysis of diagnostic accuracy using ROC curves and survival analysis with K-M plots.
• In vitro studies using human umbilical vein endothelial cells (HUVECs) to assess the impact of miR-181b-5p on cell viability, migration, apoptosis, and inflammatory responses.
• Identification and functional validation of miR-181b-5p target genes.

Main Results:

• Reduced miR-181b-5p expression was observed in DVT patients and correlated with a higher incidence of PTS.
• miR-181b-5p demonstrated significant diagnostic value for DVT (AUC=0.948).
• Overexpression of miR-181b-5p enhanced HUVEC viability and migration, suppressed apoptosis, and reduced the release of inflammatory and adhesion factors.
• HEY2 was identified as a direct target of miR-181b-5p, and its overexpression reversed the protective effects of miR-181b-5p in HUVECs.

Conclusions:

• miR-181b-5p is a promising biomarker for DVT diagnosis and predicting PTS development.
• Modulating miR-181b-5p, potentially by targeting HEY2, can mitigate endothelial cell damage in DVT.
• Further research into miR-181b-5p as a therapeutic target for DVT is warranted.