Metabolomic Analysis of HUVEC After Thermal Denaturation UHPLC-MS/MS-Based Metabolomics

Preserving denatured dermis has been shown to promote wound healing and improve skin appearance and function. Angiogenesis is crucial for the healing of burn wounds. However, the metabolic mechanisms underlying angiogenesis during burn recovery remain unclear. In this study, ultra-high performance liquid chromatography-mass spectrometry analysis revealed 6 distinct metabolites in a heat-denatured cell model. A bioinformatics approach was used to predict the differentially expressed metabolites and 4 metabolic pathways closely related to trauma repair were identified. These pathways might play a significant role in the regression of thermally injured endothelial cells. We also found that increasing D-mannose level promoted the angiogenic activity of human umbilical vein endothelial cells in the heat-denatured cell model, enhancing cell proliferation, migration, and tube formation. In summary, these findings revealed changes in metabolites and metabolic pathways in thermally injured endothelial cells and demonstrated that D-mannose could promote angiogenesis during the recovery of thermally injured endothelial cells.