Detection of antibodies to Mycoplasma felis in horses

Mycoplasma felis has been isolated from horses with pleuritis, and limited research indicates that mycoplasma pleuritis can be reproduced in horses. The serodiagnostic potential of the indirect hemagglutination and the metabolism-inhibition tests was evaluated by testing 177 horses for antibodies to M felis. Seven horses with M felis pleuritis developed antibodies, and 6 horses with sterile or bacterial pleuritis had high titers suggesting a previous M felis infection. Six horses with pleuritis (one sterile and five bacterial) had low or no titers to M felis. Only one of 30 horses with conditions other than respiratory diseases seroconverted during hospitalization and the remaining horses had low titers. Seventy-eight foals, 4 to 6 months old, from one farm did not have titers, whereas 7 out of 50 yearlings from the same farm had high titers in the indirect hemagglutination test and titers in the metabolism-inhibition test. It appears that both tests are suitable for serodiagnosis of M felis infection in horses.