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Updated: Jun 8, 2025

A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography HPLC
Published on: March 15, 2017
Herpetosin A, a nor-cucurbitane triterpenoid with a 5/6/6/5 ring system from Herpetospermum pedunculosum
Qianyu Chen1,2, Yingxiong Ma1, Zhaoyue Dong1,2
1Chongqing Key Laboratory of New Drug Screening from Traditional Chinese Medicine, Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City & Southwest University, SWU-TAAHC Medicinal Plant Joint R&D Centre, College of Pharmaceutical Sciences, Southwest University, Chongqing, P.R. China.
Abstract:
Two previously undescribed triterpenoids, herpetosin A (1) and herpetosin B (2), together with two known compounds, cucurbitacin B (3) and dihydrocucurbitacin E (4), were isolated from the seeds of Herpetospermum pedunculosum. Their structures were elucidated by analysis of the NMR, HR-ESI-MS, X-ray data and quantum chemical calculation. Compound 1 featured an unprecedented nor-cucurbitane triterpenoid skeleton with a 5/6/6/5 ring system. Among all the isolates, compound 4 exhibited anti-proliferative effect with IC50 values of 4.5 ± 0.1 μM against A549 cells, 8.2 ± 0.5 μM against MDA-MB-231 cells, and 2.4 ± 0.3 μM against HepG2 cells, respectively.
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