Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optimization of HPLC method for metanephrine and normetanephrine detection in urine: Enhancing diagnostic precision for pheochromocytoma.

Toxicology reports·2025
Same author

Hydrochemical, hydrogeological, and cancer health risk analysis of groundwater due to anthropogenic activities, Hasilpur, Pakistan.

Environmental monitoring and assessment·2024
Same author

Eco-friendly synthesis of mesoporous bioactive glass ceramics and functionalization for drug delivery and hard tissue engineering applications.

Biomedical materials (Bristol, England)·2024
Same author

Exploring the Mechanism of Anionic Chemosensing by Imidazoles: A Review.

Critical reviews in analytical chemistry·2023
Same author

Investigation and Discrimination of Ballpoint Pen Inks by Analytical Techniques and Chemometrics.

International journal of analytical chemistry·2022
Same author

Sulfonamides as Optical Chemosensors.

Critical reviews in analytical chemistry·2022

Related Experiment Video

Updated: Jun 8, 2025

GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil
11:06

GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil

Published on: May 19, 2012

16.9K

Evaluating quackery formulations: LC-MS/MS based method for detecting glucocorticoid content.

Hafiza Monaza Batool1, Muhammad Irfan Jalees2, Madeeha Batool1

  • 1School of Chemistry, University of the Punjab, Lahore 54590, Pakistan.

Toxicology Reports
|November 5, 2024
PubMed
Summary
This summary is machine-generated.

A new liquid chromatography-tandem mass spectrometry method accurately detects four glucocorticoids in unregulated medicines. This validated technique is crucial for identifying these potent anti-inflammatory drugs in quackery formulations.

Keywords:
GlucocorticoidsLiquid chromatography-tandem mass spectrometry (LC-MS/MS)Method developmentQuackery formulationsValidation

More Related Videos

Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids
08:02

Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids

Published on: April 25, 2016

9.6K
Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
14:42

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Published on: September 23, 2021

4.7K

Related Experiment Videos

Last Updated: Jun 8, 2025

GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil
11:06

GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil

Published on: May 19, 2012

16.9K
Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids
08:02

Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids

Published on: April 25, 2016

9.6K
Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
14:42

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Published on: September 23, 2021

4.7K

Area of Science:

  • Analytical Chemistry
  • Pharmacology

Background:

  • Glucocorticoids are vital anti-inflammatory drugs.
  • Unauthorized medicinal powders pose health risks due to unverified ingredients.

Purpose of the Study:

  • To develop and validate a sensitive LC-MS/MS method for simultaneously quantifying four glucocorticoids (betamethasone, dexamethasone, hydrocortisone, prednisolone).
  • To detect these glucocorticoids in unregulated medicinal powders often linked to quackery.

Main Methods:

  • Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was employed.
  • Extraction with methanol followed by chromatographic separation on an Agilent Poroshel 120 C18 column.
  • Method validation included determining the analytical measuring range (AMR), coefficient of determination (r²), accuracy, and precision.

Main Results:

  • The validated method demonstrated excellent performance with r² >0.990 for most glucocorticoids.
  • Accuracy (bias 6-15%) and precision (CV <15%) were within acceptable limits.
  • The method successfully quantified betamethasone, dexamethasone, hydrocortisone, and prednisolone in tested samples.

Conclusions:

  • The developed LC-MS/MS method is sensitive, accurate, and precise for detecting and quantifying specified glucocorticoids.
  • This validated analytical tool is valuable for identifying these drugs in unregulated quackery formulations, particularly in Pakistani contexts.