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Genome-Wide Development and Characterization of Microsatellite Markers in the Great Web-Spinning Sawfly Acantholyda posticalis

  • 0Key Laboratory of Biodiversity Conservation of National Forestry and Grassland Administration Ecology and Nature Conservation Institute, Chinese Academy of Forestry Beijing China.
Ecology and Evolution +

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November 5, 2024

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November 5, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Researchers developed 56 genome-wide microsatellite markers for the great web-spinning sawfly (Acantholyda posticalis). These markers effectively distinguish between Chinese populations and can aid in studying intraspecies variation and evolutionary history.

Area Of Science

  • Forest entomology
  • Molecular ecology
  • Population genetics

Background

  • The great web-spinning sawfly (Acantholyda posticalis) causes significant damage to Pinus forests.
  • Uncertainties exist regarding intraspecies variation and subspecies within A. posticalis.

Purpose Of The Study

  • To develop genome-wide microsatellite markers for Acantholyda posticalis.
  • To provide tools for future studies on intraspecies variation and evolutionary history.

Main Methods

  • Genome sequences were mined for microsatellite markers.
  • 56 microsatellite markers were developed and screened.
  • Marker polymorphism was assessed in Shandong (SD) and Heilongjiang (HLJ) populations.
  • Cross-amplification was performed in related Cephalcia species.
  • Population structure was analyzed using STRUCTURE and Principal Coordinate Analysis (PCoA).

Main Results

  • 56 genome-wide microsatellite markers were successfully developed for A. posticalis.
  • Markers exhibited varying levels of polymorphism (high, moderate, low).
  • Successful cross-amplification was achieved in three related Cephalcia species.
  • STRUCTURE and PCoA analyses revealed distinct genetic clusters for SD and HLJ populations, demonstrating marker resolution.

Conclusions

  • The 56 developed microsatellite markers are efficient tools for studying intraspecies variation in A. posticalis.
  • These markers can facilitate research into the evolutionary history of A. posticalis.
  • The markers also show potential for use in related species like Cephalcia.

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