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Hypericum Perforatum Callus Extract-Loaded Composite Hydrogel with Diverse Bioactivities for Enhanced Wound Healing
Tayebeh Zivari-Ghader1, Behrooz Shokouhi2, Morteza Kosari-Nasab3
1Department of Medicinal Chemistry, Faculty of Pharmacy, Tabriz University of Medical Science, Tabriz, 51664-14766, Iran.
Small (Weinheim an Der Bergstrasse, Germany)
|November 5, 2024
Summary
This study developed a novel hydrogel wound dressing using Hypericum perforatum callus extract (HPCE) to prevent skin fibrosis. The dressing promotes healing and reduces scarring by leveraging HPCE
Area of Science:
- Biomaterials Science
- Wound Healing Research
- Pharmacognosy
Background:
- Plant callus, particularly from Hypericum perforatum, yields valuable phytochemicals like hyperforin and quercetin.
- These compounds possess significant antibacterial, antioxidant, anti-inflammatory, and anti-fibrotic properties beneficial for wound repair.
- Fibrosis, a major complication of wound healing, can impair skin structure and function.
Purpose of the Study:
- To develop and evaluate a novel poly(vinyl alcohol)/chitosan/alginate (PCA) hydrogel wound dressing incorporating Hypericum perforatum callus extract (HPCE).
- To assess the dressing's efficacy in promoting wound healing and preventing fibrosis.
- To investigate the dressing's biocompatibility, antibacterial activity, and effects on tissue regeneration markers.
Main Methods:
- Fabrication of a porous PCA hydrogel wound dressing loaded with HPCE.
- In vitro assessment of biocompatibility, cell proliferation, cell adhesion, and antibacterial activity against resistant strains.
- In vivo evaluation of anti-inflammatory effects, angiogenesis, re-epithelialization, collagen deposition, and fibrosis markers (p16, p53) via immunohistochemistry.
Main Results:
- The PCA/HPCE hydrogel dressing exhibited suitable mechanical properties, high swelling capacity, biocompatibility, and promoted cell proliferation and adhesion.
- The dressing demonstrated inhibition of antibiotic-resistant bacteria and significant anti-inflammatory effects in vivo.
- In vivo studies showed enhanced angiogenesis, re-epithelialization, collagen deposition, and importantly, prevention of fibrosis as indicated by p16 and p53 marker analysis.
Conclusions:
- The developed PCA/HPCE hydrogel wound dressing is a promising therapeutic agent for effective wound healing.
- The dressing's ability to inhibit fibrosis formation post-healing offers a significant advantage over conventional treatments.
- This innovative biomaterial leverages plant-derived compounds to address critical challenges in regenerative medicine and tissue repair.
