Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Role of Matrix Metalloproteases in Degradation of ECM

Role of Matrix Metalloproteases in Degradation of ECM

Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Fecal Microbiota Transplant in Parkinson's Disease: Insights into the Gut-Brain Axis and the Challenge of Sustained Response.

Annals of neurology·2026

Same author

Attenuation of cue-induced heroin-seeking by the atypical antidepressant mirtazapine.

Drug and alcohol dependence reports·2026

Same author

Prebiotic intervention changes host and microbe proteomes in plasma extracellular vesicles of Parkinson's disease.

Scientific reports·2026

Same author

The Role of Epigenetics in Myocardial Infarction: Mechanism, Biomarkers and Therapeutic Potential.

Galen medical journal·2026

Same author

Systematic health destruction in Iran by Operation Epic Fury.

Lancet (London, England)·2026

Same author

Immune fitness and biomarkers of immune function: Relationships with the oral and gut microbiome composition.

Brain, behavior, & immunity - health·2026

Search research articles

Related Experiment Video

Updated: Jun 8, 2025

Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis

Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis

Published on: January 5, 2017

Matrix Metalloproteinase-9 Signaling Regulates Colon Barrier Integrity in Models of HIV Infection.

Michael Ohene-Nyako^1,2,3, Amanda L Persons^4,2, Christopher Forsyth^5,6,7

¹Department of Pharmacology, Rush University Medical Center, 1735 W. Harrison Street Cohn Research Building Suite #424, Chicago, IL, 60612, USA.

Journal of Neuroimmune Pharmacology : the Official Journal of the Society on Neuroimmune Pharmacology

|November 5, 2024

Summary

Human immunodeficiency virus (HIV) proteins damage the gut barrier by decreasing occludin and increasing MMP-9. Pioglitazone, a PPARγ agonist, protected against these HIV-induced gut barrier disruptions.

Keywords:

Caco-2 cells ERK HIV-1 transgenic rat NFκB PPARγ

More Related Videos

Mechanistic Insight into the Development of TNBS-Mediated Intestinal Fibrosis and Evaluating the Inhibitory Effects of Rapamycin

Mechanistic Insight into the Development of TNBS-Mediated Intestinal Fibrosis and Evaluating the Inhibitory Effects of Rapamycin

Published on: September 12, 2019

Author Spotlight: Investigating the Effects of Compounds on Intestinal Tissue Using 3D Human Cell Line Models

Author Spotlight: Investigating the Effects of Compounds on Intestinal Tissue Using 3D Human Cell Line Models

Published on: September 1, 2023

Related Experiment Videos

Last Updated: Jun 8, 2025

Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis

Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis

Published on: January 5, 2017

Mechanistic Insight into the Development of TNBS-Mediated Intestinal Fibrosis and Evaluating the Inhibitory Effects of Rapamycin

Mechanistic Insight into the Development of TNBS-Mediated Intestinal Fibrosis and Evaluating the Inhibitory Effects of Rapamycin

Published on: September 12, 2019

Author Spotlight: Investigating the Effects of Compounds on Intestinal Tissue Using 3D Human Cell Line Models

Author Spotlight: Investigating the Effects of Compounds on Intestinal Tissue Using 3D Human Cell Line Models

Published on: September 1, 2023

Area of Science:

Gastroenterology
Virology
Immunology

Background:

HIV infection compromises gut barrier integrity, leading to systemic inflammation.
HIV-1 transgenic rats exhibit disrupted colon epithelial barrier proteins.
Methamphetamine use is common in HIV-infected individuals and may worsen health outcomes.

Purpose of the Study:

To investigate mechanisms of HIV-associated gut barrier pathology.
To determine if methamphetamine exacerbates HIV-related gut issues.
To evaluate the role of HIV-1 toxic proteins and potential therapeutic interventions.

Main Methods:

Analysis of colon samples from HIV-1 transgenic and non-transgenic rats exposed to methamphetamine.
Immunoblotting for occludin and matrix metalloproteinase-9 (MMP-9).
In vitro studies using Caco-2 cells exposed to HIV-1 Tat protein and pioglitazone.

Main Results:

HIV-1 transgenic rats showed decreased occludin and increased MMP-9 levels and activity.
A significant inverse correlation was found between occludin levels and MMP-9 activity.
HIV-1 Tat protein decreased transepithelial resistance and tight junction proteins in Caco-2 cells, an effect mitigated by pioglitazone.

Conclusions:

HIV-1 proteins disrupt the gut barrier through MMP-9 dysregulation.
Low-dose methamphetamine did not significantly worsen HIV-associated gut pathology in this model.
Pioglitazone demonstrates potential in preventing and restoring HIV-induced gut barrier damage.