Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Dissecting Agronomically favorable genotypes in temperate japonica rice via haplotype analysis of a Japan-MAGIC population.

The Plant journal : for cell and molecular biology·2026

Same author

Differential regulation of heterochromatin and euchromatin by GmDDM1 during seed development ensures seedling viability in soybean.

The Plant journal : for cell and molecular biology·2026

Same author

Biomarker genes for model-based prediction of drought-stress perception levels in rice.

BMC plant biology·2025

Same author

Evaluation of transgenic rice seeds expressing T cell epitopes of Japanese cedar pollen allergens.

Transgenic research·2025

Same author

Expression and Purification of pAG-MNase for CUT&RUN.

Methods in molecular biology (Clifton, N.J.)·2024

Same author

In Vitro Genome-Wide Identification of Transcription Factor Binding Sites.

Methods in molecular biology (Clifton, N.J.)·2024

Search research articles

Related Experiment Video

Updated: May 7, 2026

Isolation of Viable Multicellular Glands from Tissue of the Carnivorous Plant, Nepenthes

Isolation of Viable Multicellular Glands from Tissue of the Carnivorous Plant, Nepenthes

Published on: December 22, 2013

Total RNA Extraction from Rice Vegetative Tissues Using Magnetic Beads.

Kanami Yoshino¹, Taiji Kawakatsu²

¹Department of Agrobiology and Bioresources, Faculty of Agriculture, Utsunomiya University, Utsunomiya, Tochigi, Japan.

Methods in Molecular Biology (Clifton, N.J.)

|November 5, 2024

Summary

This study presents HighGI, a cost-effective, high-throughput ribonucleic acid (RNA) extraction method using paramagnetic beads. HighGI enables semi-automation and preserves small RNA molecules, crucial for gene expression analysis.

Keywords:

Gene expression analysis Guanidine thiocyanate Magnetic beads Small RNA extraction Transcriptomes

More Related Videos

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

Published on: July 27, 2018

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Published on: July 9, 2021

Related Experiment Videos

Last Updated: May 7, 2026

Isolation of Viable Multicellular Glands from Tissue of the Carnivorous Plant, Nepenthes

Isolation of Viable Multicellular Glands from Tissue of the Carnivorous Plant, Nepenthes

Published on: December 22, 2013

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

Published on: July 27, 2018

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Published on: July 9, 2021

Area of Science:

Molecular Biology
Genomics
Biotechnology

Background:

Ribonucleic acid (RNA) extraction is a foundational step for gene expression analysis.
Current methods, particularly commercial column-based kits, can be expensive and may result in the loss of small RNA molecules.

Purpose of the Study:

To describe a novel, high-throughput RNA extraction method named HighGI.
To present HighGI as an inexpensive and efficient alternative for large-scale transcriptome studies.

Main Methods:

HighGI utilizes guanidine thiocyanate and isopropyl alcohol for RNA extraction.
Carboxyl-coated paramagnetic beads are employed, facilitating semi-automation with liquid handling systems.
Homemade reagent mixes are used to reduce costs.

Main Results:

The HighGI method allows for high-throughput RNA extraction suitable for large-scale transcriptome studies.
RNA extracted using HighGI retains low molecular weight RNA molecules (less than 200 bp).
The method is cost-effective due to the use of homemade paramagnetic bead and buffer mixes.

Conclusions:

HighGI offers an efficient, inexpensive, and scalable solution for RNA extraction.
The method's ability to preserve small RNA molecules makes it valuable for comprehensive gene expression analysis.