Related Experiment Video

Updated: Jun 8, 2025

In Situ Hybridization for the Precise Localization of Transcripts in Plants
12:15

In Situ Hybridization for the Precise Localization of Transcripts in Plants

Published on: November 23, 2011

51.4K

Spatial Analysis of Gene Expression by In Situ Hybridization

Masayuki Miya1, Ken-Ichiro Hibara2, Jun-Ichi Itoh3

  • 1National Institute of Genetics, Mishima, Shizuoka, Japan.

Methods in Molecular Biology (Clifton, N.J.)
|November 5, 2024
PubMed

Keywords:
DigoxigeninIn situ hybridizationNBT/BCIPRNA probeSpatial gene expression pattern

More Related Videos

Genotyping and Quantification of In Situ Hybridization Staining in Zebrafish05:41

Genotyping and Quantification of In Situ Hybridization Staining in Zebrafish

Published on: January 28, 2020

9.5K
Analysis of Single-cell Gene Transcription by RNA Fluorescent In Situ Hybridization FISH13:06

Analysis of Single-cell Gene Transcription by RNA Fluorescent In Situ Hybridization FISH

Published on: October 7, 2012

15.5K

Related Concept Videos

In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
9.2K
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
19.7K
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
17.2K