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Lasy-Seq: A High-Throughput 3' RNA-Seq Method for Large-Scale Transcriptome Profiling in Rice
Natsumi Mori-Moriyama1, Atsushi J Nagano2,3
1Faculty of Agriculture, Ryukoku University, Shiga, Japan.
Abstract:
Lasy-Seq (low-cost and easy RNA-seq) is a high-throughput library preparation method for 3' RNA-seq. Usage of oligo(dT) primers with unique index sequences allows simultaneous reverse transcription (RT) reaction and sample indexing. Therefore, each post-RT step, which was performed on a sample-by-sample basis in a conventional method, can be performed on a large number of samples together in a single tube. With the Lasy-Seq, the sequencing-ready library pool can be prepared from a few to several hundred samples in a few days.
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