Optimizing ultrasonication-assisted comprehensive extraction of bioactive flavonoids from Pterocarpus santalinus...

Area of Science:

• Phytochemistry
• Natural Product Chemistry
• Analytical Chemistry

Background:

• Pterocarpus santalinus leaves are a rich source of bioactive compounds.
• Flavonoids, particularly flavone-glycosides, exhibit significant health benefits.
• Efficient extraction methods are crucial for isolating these valuable compounds.

Purpose of the Study:

• To identify and quantify major flavone-glycosides in Pterocarpus santalinus leaves.
• To optimize ultrasonication-assisted extraction (UAE) conditions for these compounds.
• To establish a sustainable method for recovering health-beneficial flavonoids.

Main Methods:

• Untargeted metabolomics using Ultra-High-Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry (UHPLC-ESI-MSn) for compound identification.
• Response Surface Methodology (RSM) with Box-Behnken design for optimizing extraction parameters.
• Analysis of extraction time, temperature, and biomass-solvent ratio.

Main Results:

• Identification of flavone-glycosides with isorhamnetin and quercetin skeletons.
• Determination of optimal ultrasonication conditions: 19.09 min extraction time, 48.65 °C temperature, and a 72.15 biomass-solvent ratio.
• Significant correlation between optimized parameters and high extractive yield and flavonoid content.

Conclusions:

• Ultrasonication-assisted extraction is an effective method for obtaining flavone-glycosides from Pterocarpus santalinus.
• The optimized conditions provide a sustainable and efficient approach for flavonoid recovery.
• These findings support the utilization of Pterocarpus santalinus derived flavonoids in various industries.