Decreased Serum Insulin Receptor Messenger RNA Level in H. pylori IgG Seropositive Type 2 Diabetic Patients
Related Experiment Video
Updated: Jun 8, 2025
Retinal and Choroidal Thickness Changes in Populations with Helicobacter pylori Infection by Swept-Source Optical Coherence Tomography
Published on: November 1, 2024
Emmanuel Ayitey Tagoe1, Jael Acquah Appiah1, Pius Agyenim Boateng2
1Department of Medical Laboratory Sciences, University of Ghana, Accra, Ghana.
Background:
Helicobacter pylori (H. pylori) is a known gastro-intestinal pathogen but implicated in extra-gastric diseases. The relationship between H. pylori infection and type 2 diabetes (T2DM) remains insufficiently elucidated, particularly in terms of molecular mediators such as microRNAs (miRNAs) and messenger RNAs (mRNAs).
Objective:
We aimed to characterize expression pattern of insulin signalling mRNAs and targeted miRNAs in T2DM patients exposed to H. pylori infection.
Methods:
We conducted a cross-sectional study among patients diagnosed with type 2 diabetes mellitus and were aged 18 to 60 years. Overnight fasting blood samples were collected and processed for plasma and serum. The plasma samples were used for glucose estimation and the serum used for H. pylori IgG screening. Total RNA was extracted from the serum with commercial kit, and mRNAs and miRNAs quantified by RT-qPCR with specific primers and under predetermined amplification conditions. Clinical data were obtained from medical records of patients.
Results:
Among 351 patients enrolled, 267 (76.1%) were females, 224 (63.8%) were married, and 79 (22.5%) had tertiary education. Expression level of insulin receptor mRNA was significantly lower in H. pylori positive T2DM patients compared to H. pylori negative (P < .05). There was no evidence of a difference in insulin receptor substrate 1 mRNA level (P > .05). Although not statistically significant, the expression levels of miRNA-222 and miRNA-155 in the patients exposed to H. pylori were higher than that of the unexposed group (P > .05).
Conclusions:
We found a significantly reduced serum insulin receptor messenger RNA level and higher levels of miRNA-222 and miRNA-155 in H. pylori exposed T2DM patients. The findings suggest a possible role of the infection in insulin signalling alteration in the patients.
Insights
Helicobacter pylori infection in type 2 diabetes patients was linked to lower insulin receptor mRNA. This suggests H. pylori may alter insulin signaling pathways, impacting diabetes management.
Area of Science:
- Endocrinology
- Microbiology
- Molecular Biology
Background:
- Helicobacter pylori (H. pylori) is a gastrointestinal pathogen linked to various extra-gastric diseases.
- The connection between H. pylori infection and type 2 diabetes mellitus (T2DM) is not fully understood, especially regarding molecular mechanisms like microRNAs (miRNAs) and messenger RNAs (mRNAs).
Purpose of the Study:
- To investigate the expression patterns of insulin signaling-related mRNAs and specific miRNAs in T2DM patients with H. pylori infection.
Main Methods:
- A cross-sectional study involved 351 T2DM patients (aged 18-60).
- Serum samples were analyzed for H. pylori IgG antibodies, and total RNA was extracted for quantifying insulin receptor mRNA, insulin receptor substrate 1 mRNA, miRNA-222, and miRNA-155 using RT-qPCR.
- Clinical data were collected from patient records.
Main Results:
- Insulin receptor mRNA expression was significantly lower in H. pylori-positive T2DM patients compared to H. pylori-negative patients (P < .05).
- No significant difference was observed in insulin receptor substrate 1 mRNA levels (P > .05).
- While not statistically significant, miRNA-222 and miRNA-155 levels were higher in H. pylori-exposed patients.
Conclusions:
- H. pylori-exposed T2DM patients exhibited reduced serum insulin receptor mRNA levels.
- Elevated levels of miRNA-222 and miRNA-155 were observed in H. pylori-exposed T2DM patients.
- These findings suggest a potential role for H. pylori infection in altering insulin signaling pathways in T2DM.
More Related Videos
09:05High Resolution Electron Microscopy of the Helicobacter pylori Cag Type IV Secretion System Pili Produced in Varying Conditions of Iron Availability
Published on: November 21, 2014
10:34Measurement of Differentially Methylated INS DNA Species in Human Serum Samples as a Biomarker of Islet β Cell Death
Published on: December 21, 2016
Related Concept Videos
01:28Peptic Ulcer Disease III: Clinical Manifestations and Diagnostic Studies
Few clinical manifestations differentiate gastric ulcers from duodenal ulcers. Distinctions in the location, timing, and pain relief are crucial for healthcare providers in differentiating between gastric and duodenal ulcers during clinical assessments.
01:17Gastritis-II: Pathophysiology
In acute gastritis, the gastric mucosa becomes swollen and red and undergoes superficial erosion. Superficial ulceration may lead to bleeding.
In chronic gastritis, persistent or repeated insults lead to chronic inflammatory changes and, eventually, thinning or atrophy of the gastric tissue.
Gastritis can stem from various causes, each...
01:28Insulin: The Receptor and Signaling Pathways
01:23Dipeptidyl Peptidase 4 Inhibitors
01:30Peptic Ulcer Disease I: Introduction
An acute ulcer, marked by superficial erosion and minimal inflammation, swiftly resolves upon identifying and addressing the underlying cause. In contrast, a chronic ulcer persists, potentially eroding through the muscular wall and forming fibrous tissue.
Peptic ulcers can also be...
01:24Glucagon-like Receptor Agonists
GLP-1, when administered in high doses intravenously, triggers insulin secretion, inhibits glucagon release, slows gastric emptying, reduces food intake, and restores normal insulin secretion. However, its rapid inactivation by...