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Updated: Apr 30, 2026

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
Published on: September 5, 2013
Clonal distribution and molecular characterization of Staphylococcus aureus isolated strains in Chania and Heraklion,
Eftychios Vittorakis1,2,3, Mihaela Laura Vica2, Stanca-Lucia Pandrea1,4
1Department of Microbiology, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Aim:
This study investigates the demographic distribution, antibiotic resistance profiles, and molecular characteristics of Staphylococcus aureus infections.
Methods:
The study was carried out in 141 patients, 60.4% male, in patients from Chania and Heraklion, Crete.
Results:
The highest infection prevalence observed in the older adults (≥65 years) age group. The predominant infection types were skin lesions (39.72) and respiratory tract infection (22.7%). Antibiotic resistance testing revealed that 57.44% of strains were MRSA, with high resistance to Tetracycline, Ciprofloxacin, Kanamycine Erythromycin and Clindamycin. Molecular analysis showed 19.14% of strains were Pvl-positive, highlighting the presence of both MRSA and MSSA strains with Pvl genes.
Conclusions:
The study underscores the need for continuous surveillance and targeted infection control strategies to manage the spread of MRSA, particularly in vulnerable populations.
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