Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

3.1K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
3.1K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.1K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.1K
Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

9.9K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
9.9K
COP Coated Vesicles00:59

COP Coated Vesicles

7.7K
Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of...
7.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Association between first-trimester chorionic bump and early pregnancy loss: a two-center retrospective study with exploratory radiomics analysis.

BMC medical imaging·2026
Same author

Molecular PET imaging of integrin αvβ6 reveals biliary and fibrotic changes in preclinical MASH.

Biomarker research·2026
Same author

A [<sup>68</sup>Ga]-labeled PDGFRβ-targeting peptide PET probe for assessing MASH-related fibrosis in diet-induced preclinical models and human liver specimens.

Biochemical pharmacology·2026
Same author

A Chromosome-Level Genome Assembly and Annotation of the Chinese Porcupine (<i>Hystrix hodgsoni</i>) Reveals the Expansion of Olfactory-Related Gene Families.

Genes·2026
Same author

Seasonal dynamic modeling for real-time prediction of human brucellosis epidemiological trends in Gansu, Guangdong and Sichuan Provinces, China.

PLoS neglected tropical diseases·2026
Same author

Neuroligin1 in CCK-interneurons gates social memory formation via a disinhibitory microcircuit in the hippocampal CA2 in male mice.

Nature communications·2026

Related Experiment Video

Updated: Jun 8, 2025

Super-resolution Imaging of Neuronal Dense-core Vesicles
09:30

Super-resolution Imaging of Neuronal Dense-core Vesicles

Published on: July 2, 2014

9.7K

Genetically-encoded markers for confocal visualization of single dense core vesicles.

Junwei Yu1,2, Yunpeng Zhang1,3,2, Kelsey Clements1

  • 1Department of Biology, Volen National Center for Complex Systems, Brandeis University, Waltham, MA 02454-9110, USA.

Research Square
|November 6, 2024
PubMed
Summary

Researchers developed new genetically-encoded markers to study neuronal dense core vesicles (DCVs). This allows for single-vesicle analysis using confocal microscopy, overcoming electron microscopy limitations.

Keywords:
Drosophilaco-transmissionconfocal microscopyexpansion microscopylarge dense core vesiclepeptide modulatorsvesicle markers

More Related Videos

Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions
09:36

Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions

Published on: August 26, 2021

3.8K
Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis
08:32

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

Published on: February 14, 2022

7.6K

Related Experiment Videos

Last Updated: Jun 8, 2025

Super-resolution Imaging of Neuronal Dense-core Vesicles
09:30

Super-resolution Imaging of Neuronal Dense-core Vesicles

Published on: July 2, 2014

9.7K
Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions
09:36

Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions

Published on: August 26, 2021

3.8K
Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis
08:32

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

Published on: February 14, 2022

7.6K

Area of Science:

  • Neuroscience
  • Cell Biology
  • Molecular Biology

Background:

  • Neuronal dense core vesicles (DCVs) are crucial for storing and releasing various neuromodulators, trophic factors, and bioamines.
  • Analyzing single DCVs is challenging with traditional electron microscopy, hindering the understanding of cargo segregation and vesicle heterogeneity.

Purpose of the Study:

  • To overcome the limitations of electron microscopy for single DCV analysis.
  • To develop novel tools for visualizing and analyzing DCV heterogeneity and cargo segregation at single-vesicle resolution.

Main Methods:

  • Development of genetically-encoded markers specifically designed for DCVs.
  • Integration of these markers with standard immunohistochemistry techniques.
  • Application of expansion microscopy to achieve super-resolution imaging.
  • Utilizing confocal microscopy for single-vesicle analysis.

Main Results:

  • Successful development and validation of genetically-encoded markers for DCVs.
  • Demonstration of single-vesicle resolution for DCV analysis using confocal microscopy.
  • Enabled detailed investigation of DCV cargo segregation and heterogeneity.

Conclusions:

  • The new genetically-encoded markers provide a powerful approach to study neuronal DCVs.
  • This method overcomes previous limitations, allowing for unprecedented insights into DCV function and composition.
  • Facilitates advanced research in neurobiology and molecular medicine.