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Updated: Jun 8, 2025

Super-resolution Imaging of Neuronal Dense-core Vesicles
Published on: July 2, 2014
Genetically-encoded markers for confocal visualization of single dense core vesicles
Junwei Yu1,2, Yunpeng Zhang1,3,2, Kelsey Clements1
1Department of Biology, Volen National Center for Complex Systems, Brandeis University, Waltham, MA 02454-9110, USA.
Abstract:
Neuronal dense core vesicles (DCVs) store and release a diverse array of neuromodulators, trophic factors and bioamines. The analysis of single DCVs has largely been possible only using electron microscopy, which makes understanding cargo segregation and DCV heterogeneity difficult. To address these limitations, we developed genetically-encoded markers for DCVs that can be used in combination with standard immunohistochemistry and expansion microscopy, to enable single-vesicle resolution with confocal microscopy.
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