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Immunocytochemistry and Immunohistochemistry

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
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Related Experiment Video

Updated: Jun 8, 2025

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

Mechanical Microvibration Device Enhancing Immunohistochemistry Efficiency.

Weifeng Zhang¹, Jirui Li¹, Fengshan Xie¹

¹Laboratory of Molecular Pathology Department of Pathology and Pathophysiology Shantou University Medical College Shantou China.

Engineering in Life Sciences

|November 6, 2024

Summary

Mechanical microvibration (MMV) significantly speeds up immunohistochemistry (IHC) staining, reducing antibody use by over 10x. This novel technique enhances staining quality and offers substantial time and cost savings for pathology research.

Keywords:

antibody immunohistochemistry mechanical microvibration

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Last Updated: Jun 8, 2025

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Area of Science:

Biomedical research
Pathology
Biotechnology

Background:

Immunohistochemistry (IHC) is crucial for diagnostics and research.
Current IHC methods require lengthy processes and high antibody costs.
There is a need for more efficient and cost-effective IHC techniques.

Purpose of the Study:

To evaluate a novel IHC technique using mechanical microvibration (MMV).
To assess MMV's impact on staining duration, antibody consumption, and quality.
To investigate the potential clinical and financial benefits of MMV-enhanced IHC.

Main Methods:

Mechanical microvibration (MMV) was applied to tissue sections using coin vibration motors.
Multiple antibodies were tested on cancerous and normal tissues with and without MMV.
Incubation times, temperatures, and antibody dilutions were optimized.

Main Results:

MMV significantly reduced IHC staining time.
Antibody consumption was decreased by over 10-fold compared to controls.
MMV enhanced specific staining quality, resulting in stronger, better-defined reactions.
Results were validated via multicenter, double-blind assessment and quantitative image analysis.

Conclusions:

MMV offers a faster, more cost-effective IHC method.
The technique improves staining specificity and quality.
MMV accelerates antibody diffusion, leading to significant time and cost savings in diagnostic pathology and research.