Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Shared inflammatory architecture and therapeutic tensions between psoriasis and Crohn's disease.

Frontiers in immunology·2026
Same author

Electronic health record-derived care-exposure burden and central line-associated bloodstream infection among critically ill adults: a multi-source retrospective cohort study.

Frontiers in public health·2026
Same author

A Gaussian-based planning approach for robust dose-escalated stereotactic body proton therapy.

International journal of radiation oncology, biology, physics·2026
Same author

Regulatory role of epigenetics in rice immunity against bacterial and fungal pathogens.

Crop health·2026
Same author

A prospective non-randomized controlled study of a flexible panoramic electronic hysteroscope in outpatient hysteroscopic diagnosis and treatment.

European journal of obstetrics, gynecology, and reproductive biology·2026
Same author

Association between concomitant ACEI/ARBs use and survival of patients with prostate cancer receiving abiraterone acetate: A post-hoc analysis of two randomized trials.

Prostate cancer and prostatic diseases·2026

Related Experiment Video

Updated: Jul 23, 2026

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
08:25

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

Published on: April 8, 2017

13.8K

A simple, cost-effective, and efficient method for screening CRISPR/Cas9 mutants in plants.

Yiping Wang1, Jun Ma1, Yingying Wu1

  • 1College of Plant Protection, Yangzhou University, Yangzhou, Jiangsu, China.

Journal of Plant Physiology
|November 6, 2024
PubMed
Summary

We developed a new method, PCR-Bsl I-associated analysis (PCR-BAA), for quickly and affordably screening clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) genome editing mutants in plants. This technique efficiently identifies mutants, even from low-efficiency editing events.

Keywords:
ArabidopsisBsl ICRISPR/Cas9Mutant screeningRice

More Related Videos

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

12.5K
A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
08:37

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

7.5K

Related Experiment Videos

Last Updated: Jul 23, 2026

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
08:25

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

Published on: April 8, 2017

13.8K
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

12.5K
A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
08:37

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

7.5K

Area of Science:

  • Plant Biotechnology
  • Molecular Biology
  • Genetics

Background:

  • The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system is a powerful tool for targeted mutagenesis in plants.
  • Efficient screening of CRISPR/Cas9-induced mutants is crucial, particularly for low-efficiency editing events, but existing methods can be time-consuming, costly, or inefficient for large sample sizes.

Purpose of the Study:

  • To develop a rapid, cost-effective, and sensitive screening method for CRISPR/Cas9 mutants in plants.
  • To streamline the process of identifying mutants, especially from large populations and low-efficiency editing events.

Main Methods:

  • Developed and validated the PCR-Bsl I-associated analysis (PCR-BAA) method.
  • Utilized standard Polymerase Chain Reaction (PCR) and Bsl I restriction enzyme digestion.
  • Analyzed results using agarose gel electrophoresis.

Main Results:

  • The PCR-BAA method provides a simple, rapid, and low-cost approach for screening CRISPR/Cas9 mutants.
  • The method demonstrates high sensitivity, making it suitable for identifying mutants from low-efficiency editing events.
  • PCR-BAA is particularly effective for screening large populations of transformants.

Conclusions:

  • PCR-Bsl I-associated analysis (PCR-BAA) offers a significant improvement for screening CRISPR/Cas9 genome editing mutants in plants.
  • The method's efficiency, low cost, and simplicity make it ideal for high-throughput mutant screening, especially in research settings with limited resources or large experimental scales.