Establishment of continuous flow partial denitrification biofilm module with short hydraulic retention time

Partial denitrification (PD) can supply essential nitrite (NO₂^-) and is supposed to promote the application of Anammox. However, PD-related research mainly involves sequencing batch reactors and activated sludge. Here, we proposed establishing PD in a continuous-flow submerged biofilm module (PD-BfM). Benefiting from employing anoxic starvation treatment to quickly start PD and transferring enriched functional bacteria onto biofilms in time, the preparation work of PD-BfM was completed within a quite short period of 21 days. With the hydraulic retention time adjusted to 50 min, PD-BfM demonstrated an impressive efficiency in generating NO₂^-, achieving a nitrate-to-nitrite transformation ratio of over 75 %, even at the influent chemical oxygen demand to nitrate ratio of 4 condition. Meanwhile, the dominant genus in the biofilms was shifted from Thauera to Flavobacterium and Comamonadaceae family members. The gradient of substrate concentrations also possibly differentiated microbial communities between the top and bottom bio-carriers.